How to Do the Vitamin D 25 Hydroxy Test?

Vitamins can be tested by liquid chromatography-tandem mass spectrometry, enzyme-linked immunosorbent assay, high-performance liquid chromatography, chemiluminescence method, etc. The vitamin D 25 hydroxy test methods are as follows:

1. Liquid chromatography-tandem mass spectrometry of vitamin D 25 hydroxy test

Liquid chromatography-tandem mass spectrometry is a test method by liquid chromatography-tandem mass spectrometer. After the sample is processed, it is separated by liquid chromatography. In the multiple reaction monitoring scanning mode, 25(OH)D2 and 25(OH)D3 can be simultaneously tested. This test method has high sensitivity and accuracy, and is the "gold standard" for the test of vitamin b 25 hydroxyl.

2. Enzyme-linked immunosorbent assay for vitamin D 25 hydroxy test

The enzyme-linked immunosorbent assay is to combine the antigen or antibody in the serum with the enzyme complex, and to quantitatively determine the level of 25 OHD vitamin D and other hydroxylated metabolites in human serum or plasma through chromogenic detection, incubation, plate washing, substrate addition and other processes.

3. High performance liquid chromatography of vitamin D 25 hydroxy test

High performance liquid chromatography uses the selective distribution of different substances in different states, and elutes the mixture in the mobile phase relative to the stationary phase. Different substances in the mixture will move along the stationary phase at different speeds, and finally achieve the separation effect, which can reflect the level of vitamin D in the human body and is an important indicator for evaluating vitamin D.

4. Chemiluminescence method of vitamin D 25 hydroxy test

Chemiluminescence method uses chemiluminescence to determine catalysts, sensitizers, and reactants in chemiluminescence reactions and coupling reactions. This method can detect the concentration of serum 25(OH)D, and make an evaluation on the nutritional status of human vitamin D according to the measurement results.

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