C-Reaction Protein (CRP)

C-reactive protein (CRP) refers to several proteins (acute proteins) that rise sharply in blood plasma in response to infection or tissue damage.

CRP can activate complement and strengthen the phagocytosis of phagocytes and play an optimizing role, thus removing pathogenic microorganisms and damaged, necrotic, and apoptotic tissue cells from the body and playing an essential protective role in the natural immune process of the body.

C-reactive protein is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells.

A CRP test measures the amount of CRP in the blood plasma samples to detect inflammation due to acute conditions or to monitor disease severity in chronic conditions.

The performance date of hsCRP&CRP Rapid Quantitative Test Kit (FIA)

Manufacturer	SEKBIO
Parameter	hsCRP&CRP Rapid Quantitative Test Kit (FIA)
Linearity	0.5-200mg/L
Detection limit	0.5mg/L
Benchmark product	Siemens

Standard Curve

Cubic spline interpolation

Concentration (mg/L)	0	0.50	2.54	14.75	37.75	72.9	109.18	159.63	201.03
T/C	0.04707	0.12413	0.21857	0.34509	0.63201	1.16325	1.83936	3.12105	5.61028

1 Limit of detection

Result(T/C)	Concentration
Result(T/C)	0	0.50
1	0.04716	0.11718
2	0.04720	0.11303
3	0.04754	0.12718
4	0.04662	0.13166
5	0.04742	0.11821
6	0.04727	0.11744
7	0.04691	0.11337
8	0.04691	0.12607
9	0.04719	0.12536
10	0.04743	0.13306
11	0.04739	0.13173
12	0.04714	0.13180
13	0.04670	0.13457
14	0.04707	0.13098
15	0.04720	0.11420
16	0.04666	0.11822
17	0.04713	0.13125
18	0.04661	0.11592
19	0.04683	0.12039
20	0.04667	0.12609
AVE	0.04705	0.12389
SD	0.00030	/
AVE+2*SD	0.04764	/
regression equation	y=0.15367x+0.04705
LoB(mg/L)	0.00387

2 Linear

Cal	Concentration	Result	AVERAGE
S1	0.48	0.56	0.49
		0.44
		0.47
S2	2.22	1.92	2.04
		2.02
		2.18
S3	10.15	9.15	9.76
		10.11
		10.03
S4	49.83	54.02	53.11
		54.66
		50.64
S5	95.68	95.12	93.49
		95.28
		90.07
S6	205.76	201.51	215.47
		233.8
		211.11

3 Accuracy

Cal	concentration	Result	Bias
A1	2.22	2.27	2.3%
		2.17	-2.2%
		2.29	3.3%
A2	10.15	10.76	6.0%
		9.61	-5.3%
		9.73	-4.1%
A3	49.83	46.73	-6.2%
		52.54	5.4%
		47.73	-4.2%

4 Precision

Cal	Concentration	Cal	Concentration	Cal	Concentration
R1	1.93	R2	8.98	R3	52.40
	2.36		11.21		46.24
	2.39		10.74		47.25
	2.54		10.26		51.11
	2.28		11.05		49.07
	2.07		9.65		42.97
	2.29		9.73		51.22
	2.49		11.54		50.70
	2.06		9.69		43.00
	2.36		10.67		47.85
AVE	2.28	AVE	10.35	AVE	48.18
SD	0.20	SD	0.82	SD	3.36
CV	8.7%	CV	7.9%	CV	7.0%

5 Method Comparison

Sample	Siemens	Sekbio	Sample	Siemens	Sekbio
1	58	51.22	31	93.39	93.60
2	163.65	153.36	32	15.1	14.72
3	13.88	11.51	33	7.81	8.62
4	186.9	168.69	34	106.96	117.75
5	5	5.48	35	111.02	106.05
6	7.85	8.01	36	1.55	1.41
7	1.06	0.97	37	22.71	25.76
8	3.24	2.84	38	7.26	8.65
9	48.47	46.13	39	9.15	9.86
10	31.14	30.68	40	5.27	4.48
11	3.53	3.99	41	12.82	13.49
12	7.53	6.04	42	74.49	73.08
13	13.51	15.23	43	1.38	1.20
14	1.73	1.86	44	113.53	124.72
15	80.89	86.26	45	57.31	53.95
16	59.15	61.25	46	6.56	5.75
17	137.13	129.53	47	4.41	4.44
18	83.97	84.23	48	86.89	92.09
19	38.72	46.81	49	7.29	7.04
20	1.98	2.38	50	1.25	1.24
21	53.24	48.34	51	5.84	6.00
22	53.04	55.65	52	95.4	79.85
23	13.87	16.39	53	3.21	2.68
24	4.71	4.12	54	6.12	6.08
25	1.69	1.47	55	126.78	130.38
26	72.11	69.67	56	23.16	22.96
27	18.21	22.27	57	0.93	0.80
28	85.42	93.52	58	4.54	4.30
29	0.72	0.77	59	1.81	1.92
30	23.75	22.93	60	59.21	68.00

C-Reaction Protein (CRP) Products

Antibody	Application
Mouse Anti-human CRP mAb	For immunodiagnostic: ELISA, LFA, CLIA

CRP Intro

C-reactive protein (CRP) refers to some proteins (acute proteins) that rise sharply in plasma when the body is infected or tissue damaged. CRP can activate complement and strengthen the phagocytosis of phagocytes to play an opsonizing effect, thereby removing pathogenic microorganisms and damaged, necrotic, and apoptotic tissue cells that invade the body and play an important protective role in the body's natural immune process.

CRP Antibody

CRP is a non-specific inflammatory marker and directly involved in cardiovascular diseases such as inflammation and atherosclerosis and is the most potent predictor and a risk factor for cardiovascular diseases. The interaction of CRP with complement C1q and FcTR enables it to exhibit many biological activities, including host defense against infection, phagocytosis, and regulation of inflammatory responses. Binds to damaged cells, apoptotic cells, and nuclear antigens, making it also play an important role in autoimmune diseases.

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