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N-terminal Pro B Type Natriuretic Peptide (NT-proBNP)

NT-proBNP is a product released under the pressure load of the heart, and its level can reflect the myocardial function and damage degree, which is related to the severity of heart failure. It is one of the most clinically used heart failure biomarkers.


NT-proBNP has essential value in the clinical diagnosis, risk stratification, prognosis evaluation, and curative effect monitoring of patients with acute heart failure (AHF) and chronic heart failure (CHF).


This precursor molecule is cleaved to form the active BNP and the inactive N-terminal pro-BNP (NT-proBNP) fragment. Myocardial cells secreted NT-proBNP in response to increase volume and pressure. Comparisons of BNP and NT-proBNP have shown that both eight molecules effectively diagnose left ventricular dysfunction in the acute care/emergency setting.

N-terminal Pro B Type Natriuretic Peptide (NT-proBNP) Products


AntibodyApplication
Sheep anti-human NT-proBNP mAb

For immunodiagnostic: ELISA, LFA, CLIA

Mouse anti-human NT-proBNP mAb
Humanized anti-human NT-proBNP mAb


Antigen

Application

Recombinant human NT-proBNP antigen

For immunodiagnostic: ELISA, LFA, CLIA


NT-proBNP Intro

Serum marker detection is an important means for the early diagnosis and prognosis of heart failure in clinical practice. The heart failure guidelines recommend the preferred serum markers for heart failure. Among them, natriuretic peptides (a neuroendocrine system activation marker), including brain natriuretic peptide (BNP) and amino-terminal brain natriuretic peptide (NT-proBNP), are the most popular at home and abroad (ECS/ACC/AHA/HFSA/CSC). It is recommended to use natriuretic peptides to identify high-risk heart failure groups and comprehensively manage and prevent them.


Compared with BNP, NT-proBNP has a longer half-life and better stability, so NT-proBNP has higher sensitivity for detecting early or mild heart failure, and it takes more time for blood samples to be sent to the laboratory making it possible more suitable for clinical applications.

NT-proBNP Rapid POCT Test

Because NT-proBNP has a long half-life and is relatively more stable, the specimens can be stored and detected either in the central laboratory or at the bedside using the NT-proBNP Rapid POCT test, while BNP has a short half-life and poor stability and can only be detected immediately at the bedside. NT-proBNP is slowly cleared in the blood and can accumulate at a higher concentration, reflecting the state and changes of cardiac function more sensitively to a certain extent, especially in the early diagnosis of heart failure. In addition, NT-proBNP has no physiological activity and is not affected by recombinant human brain natriuretic peptides (such as neoactivin). For patients treated with recombinant human brain natriuretic peptides, it is recommended to prefer NT-proBNP to evaluate the changes in cardiac function. BNP is cleared by binding to BNP clearance receptors neutral endonucleases, and a small part of it is emptied by renal metabolism. NT-proBNP is mainly removed by renal metabolism, so its concentration is more significantly affected by renal function than BNP.

NT-proBNP Performance Data

Manufacturer

SEKBIO

Parameter

NT-proBNP

Linearity

5-30000pg/ml

Detection limit

5pg/ml

Benchmark product

Roche

Test principle and procedure

NT-proBNP: Add 20 μl of sample + 50 μl of Ra +30 μl of Rd and incubation for 15 minutes. Wash with 200 μl of washing solution. Finally, add 100 μl of A + 100 μl of B.


Precision

Sample

RLU

Concentration

Sample

RLU

Concentration






302.29

10,257

289.22






4374.48

441,537

4274.56

9,994

283.88

437,911

4248.42

9,839

280.71

424,117

4148.59

10,520

294.52

453,288

4358.98

10,602

296.16

428,601

4181.11

10,660

297.32

419,557

4115.45

10,198

288.02

444,986

4299.38

10,110

286.24

409,947

4045.36

10,820

300.52

445,647

4304.14

10,659

297.30

428,028

4176.96

AVE

10365.90

291.39

AVE

433361.90

4215.30

SD

329.91

6.66

SD

13523.79

97.84

CV

3.2%

2.3%

CV

3.1%

2.3%


Limit of detection

Sample

RLU

Concentration





Ag Diluent

156

165

1.88

2.58

145

165

2.60

2.10

143

118

2.15

2.10

138

152

2.17

2.84

125

134

1.16

2.10

166

144

1.60

1.70

129

121

2.58

2.10

155

127

1.60

2.60

144

139

0.23

2.60

160

137

1.68

2.10

AVE

143.15

2.03

SD

14.92

0.60

AVE+2*SD

3.23


Linear

Sample(pg/ml)

Dilution ratio

Concentration value

Average

30095.57

1.0

29812.54

30059.12

30209.24

30095.57

27086.01

0.9

26233.17

26171.99

26785.71

26395.57

24076.46

0.8

23623.09

23707.83

21225.84

22828.49

21066.90

0.7

19671.67

19893.97

17831.12

19118.12

18057.34

0.6

15081.66

15737.83

15321.30

16379.25

15047.79

0.5

14243.18

14338.76

14074.32

14316.57

12038.23

0.4

10948.01

10825.36

11440.97

11070.93

9028.67

0.3

8359.06

8250.77

7832.37

8147.52

6019.11

0.2

5527.01

5247.89

5334.36

5370.02

3009.56

0.1

2718.47

2651.98

2688.95

2686.51

0.00

0.0

0.00

0.00

0.00

0.00



2.jpg


Method Comparison

roche-sekbio.jpg


Reagents Stability

Reagent

Sample(pg/ml)

Average

Deviation



2-8°

30,000

4,476,786


100

5,642


Antigen diluent

414


calf serum

626


random sample

1,360




37℃ 1d

30,000

4,552,605

1.69%

100

5,591

-0.90%

Antigen diluent

444

7.17%

calf serum

530

-15.43%

random sample

1,386

1.94%



37℃ 4d

30,000

4,399,886

-1.72%

100

5,159

-8.55%

Antigen diluent

455

9.98%

calf serum

686

9.47%

random sample

1,602

17.82%



37℃ 7d

30,000

4,264,465

-4.74%

100

5,361

-4.97%

Antigen diluent

460

11.11%

calf serum

634

1.22%

random sample

1,585

16.57%


Calibrator Stability

Reagent

Sample(pg/ml)

Average

Deviation

-20℃

30,000

4,541,204


1,000

81,101


100

5,458


0

443


2-8℃

30,000

4,526,167

-0.33%

1,000

80,238

-1.06%

100

5,657

3.65%

0

475

7.14%

37℃ 1d

30,000

4,525,929

-0.34%

1,000

80,476

-0.77%

100

5,617

2.91%

0

433

-2.41%

37℃ 4d

30,000

4,417,530

-2.72%

1,000

79,218

-2.32%

100

5,115

-6.28%

0

485

9.40%

37℃ 7d

30,000

4,417,530

-2.72%

1,000

78,458

-3.26%

100

5,226

-4.25%

0

418

-5.64%


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