INS(Insulin)

Introduction:

Insulin is a hormone produced by the pancreas, and it plays a crucial role in regulating glucose (sugar) metabolism in the body. The primary function of insulin is to facilitate the uptake of glucose by cells, where it can be used for energy. Insulin helps maintain optimal blood sugar levels by promoting the storage of excess glucose in the liver and muscles, preventing it from accumulating in the bloodstream.

In the realm of diabetes management and metabolic health, insulin plays a pivotal role in regulating blood sugar levels. Insulin tests are essential diagnostic tools that provide valuable insights into an individual's insulin production and help in assessing overall metabolic health. In this article, we will delve into the significance of insulin tests, their purpose, and what the results may indicate.

Clinical Significance of Insulin Test:

1. Diagnosis of Diabetes

2. Monitoring Diabetes Treatment

3. Assessment of Insulin Resistance

4. Evaluation of Pancreatic Function

5. Hypoglycemia Investigations

6. Polycystic Ovary Syndrome (PCOS) Diagnosis

The performance data of the INS Rapid Quantitative Test Kit(CLIA)

Manufacturer	SEKBIO
Parameter	INS
Linearity	0.2-1000uIU/mL
Detection limit	0.01uIU/mL

Six point calibration test results

Sample Concentration ( uIU/ml)	RLU			Average	CV	S/N
1,014.31	1,172,979	1,050,097	1,151,211	1,124,762	5.83%	962.43
200.09	204,519	236,269	200,489	213,759	9.17%	182.91
20.85	20,916	21,322	23,821	22,020	7.14%	18.84
1.95	3,033	2,775	3,191	3,000	7.00%	2.57
0.22	1,485	1,460	1,402	1,449	2.94%	1.24
0.02	1,124	1,158	1,224	1,169	4.35%	1.00

Precision

Sample	RLU	Concentration	Sample	RLU	Concentration
0.25	3,048	2.00	15.21	196,412	184.18
	3,002	1.96		216,766	202.86
	3,076	2.03		221,674	207.36
	3,191	2.15		225,286	210.67
	3,057	1.98		206,126	193.10
	3,053	2.01		195,386	183.24
	3,245	2.21		190,951	179.16
	3,269	2.24		226,027	211.35
	3,064	2.02		226,762	212.02
	3,409	2.38		210,788	197.38
AVE	3,141	2.10	AVE	211,618	198.13
SD	131.00	0.14	SD	13760.15	12.63
CV	4.17%	6.67%	CV	6.50%	6.37%

Limit of detection

Sample	RLU		Concentration
Ag Diluent	1,222	1,206	0.07	0.06
	1,231	1,267	0.08	0.09
	1,273	1,117	0.13	0.03
	1,270	1,171	0.09	0.03
	1,110	1,134	0.03	0.03
	1,141	1,331	0.03	0.19
	1,161	1,176	0.00	0.04
	1,223	1,279	0.07	0.13
	1,129	1,123	0.03	0.03
	1,171	1,262	0.04	0.14
AVE	1,200		0.07
SD	65.51		0.05
AVE+2*SD	0.164

Linear

Sample（uIU/ml)	Dilution ratio	Concentration value			Average
1014.31	1.0	1056.80	948.51	1037.63	1014.31
811.49	0.8	767.04	753.74	760.49	760.42
608.67	0.6	552.77	518.70	582.22	551.23
405.86	0.4	342.35	381.00	354.64	359.33
203.04	0.2	224.90	194.47	199.70	206.36
0.22	0.0	0.24	0.22	0.21	0.22

Method Comparison

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