Follicle Stimulating Hormone(FSH)
The performance data of the FSH rapid Quantitative Test Kit (FIA)
Manufacturer | SEKBIO |
Parameter | FSH |
Linearity | 0.1~200 mIU/ml |
Detection limit | 0.1 mIU/ml |
benchmark product | Roche |
Test principle and procedure | FSH: Add 30 μl of sample + 30 μl of Ra+ 30 μl of Rd incubation for 10 minutes. Wash with 200 μl of washing solution. Finally, add 100 μl of A + 100 μl of B. |
Six point calibration test results
Sample Concentration( mIU/ml ) |
RLU | Average | CV | S/N | ||
0.00 | 270 | 181 | 124 | 192 | 38.39% | / |
5.28 | 328,441 | 310,837 | 325,368 | 321,549 | 2.92% | 1677.65 |
45.08 | 2,734,548 | 2,761,887 | 2,619,552 | 2,705,329 | 2.79% | 14114.76 |
85.33 | 4,740,189 | 4,869,826 | 4,845,539 | 4,818,518 | 1.43% | 25140.09 |
123.20 | 6,847,928 | 6,331,928 | 6,490,393 | 6,556,750 | 4.03% | 34209.13 |
208.30 | 10,343,008 | 9,995,892 | 9,948,756 | 10,095,885 | 2.13% | 52674.18 |
Precision
Sample | RLU | Concentration | Sample | RLU | Concentration |
45.08 | 40.6 | 2,429,100 |
125.54 | 123.5 | 6,636,529 |
45.9 | 2,732,893 | 121.6 | 6,551,933 | ||
45.6 | 2,712,839 | 123.6 | 6,644,149 | ||
44.4 | 2,643,743 | 125.7 | 6,734,753 | ||
44.6 | 2,654,937 | 125.7 | 6,734,422 | ||
45.1 | 2,688,235 | 126.9 | 6,790,605 | ||
43.2 | 2,579,281 | 130.0 | 6,925,497 | ||
46.3 | 2,752,775 | 124.8 | 6,694,353 | ||
43.0 | 2,565,633 | 133.4 | 7,077,517 | ||
43.0 | 2,568,870 | 132.3 | 7,026,385 | ||
AVE | 44.17 | 2,632,830.60 | AVE | 126.74 | 6,781,614.30 |
SD | 1.74 | 98,577.72 | SD | 3.92 | 174,162.04 |
CV | 3.9% | 3.7% | CV | 3.1% | 2.6% |
1 Limit of detection
Sample | RLU | Concentration | ||
Ag Diluent | 297 | 255 | 0.003 | 0.003 |
230 | 174 | 0.002 | 0.002 | |
181 | 183 | 0.002 | 0.003 | |
216 | 158 | 0.002 | 0.002 | |
195 | 132 | 0.002 | 0.000 | |
267 | 135 | 0.003 | 0.000 | |
152 | 128 | 0.002 | 0.000 | |
191 | 113 | 0.002 | 0.000 | |
178 | 166 | 0.001 | 0.002 | |
192 | 206 | 0.002 | 0.002 | |
AVE | 210 | 0.001 | ||
SD | 48 | 0.0005 | ||
AVE+2*SD | 0.002 | |||
2 Linear
Sample( mIU/ml ) | Dilution ratio | Concentration value | Average | ||
0.00 | 0.0 | 0 | 0 | 0 | 0 |
41.85 | 0.2 | 51.68 | 51.02 | 50.81 | 51 |
83.69 | 0.4 | 95.49 | 98.25 | 90.87 | 95 |
125.54 | 0.6 | 136.10 | 135.28 | 135.00 | 135 |
167.39 | 0.8 | 176.07 | 177.51 | 174.82 | 176 |
209.24 | 1.0 | 206.04 | 211.97 | 209.70 | 209.24 |
3 Method Comparison
- Cardiac Markers
-
Tumor Marker
- CA50
-
CA125
- CA242
-
CA15-3
- CA19-9
- CA72-4
-
Pepsinogens I (PGI)
-
Human Epididymis 4 (HE4)
- Prostate-Specific Antigen (PSA)
- Squamous Cell Carcinoma (SCC)
- Neuron-Specific Enolase (NSE)
- Cytokeratin 19 Fragment (CYFRA21-1)
- Human Progastrin-releasing Peptide (ProGRP Tumor Marker)
- Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA II Tumor Marker)
- Alpha-fetoprotein(AFP)
- Inflammatory Marker
- Infectious Disease
- Hormones
- Thyroid Function
- Glucose Metabolism
- Bone Marker
- Others
-
Heterophilic Blocking Reagent
- Animal Diagnostics
