G17
Gastrin G17 is a vital gastrointestinal hormone, primarily secreted by G cells in the antrum of the stomach. It plays a crucial role in regulating gastrointestinal functions, particularly in modulating gastric acid secretion and maintaining the gastric mucosa.
Below are key points regarding Gastrin G17:
Biological Functions:
Promotes Gastric Acid Secretion: Gastrin G17 binds to receptors on enterochromaffin-like (ECL) cells and parietal cells, stimulating the release of histamine and other stimulants, thereby increasing gastric acid secretion. It can also transiently inhibit acid secretion through the regulation of transforming growth factor beta and related peptides.
Cell Proliferation and Differentiation: Gastrin G17 promotes the proliferation of gastric mucosal cells and inhibits apoptosis, which is vital for maintaining the integrity of the gastrointestinal mucosa.
Digestive Tract Function Regulation: It participates in regulating the function of the lower esophageal sphincter and the overall function of the digestive tract.
Clinical Significance:
Disease Diagnosis and Monitoring: Variations in Gastrin G17 levels can assist in diagnosing various gastric diseases, such as gastric and duodenal ulcers, type A atrophic gastritis (linked to Helicobacter pylori infection), gastrinomas, and hyperplasia of the antral mucosa. Abnormal Gastrin G17 levels, defined as [specify threshold], may indicate these conditions.
Gastric Cancer Screening: Gastrin G17 is considered a core indicator in early gastric cancer screening in China due to its abnormal levels in certain cases of gastric cancer. Elevated levels may suggest inflammation or other issues within the stomach, correlating with an increased risk of gastric cancer.
Decision Support: Gastrin G17 testing can serve as an initial screening tool in the absence of gastroscopic examination, aiding physicians in deciding whether further gastric investigations are necessary.
Impact on Renal Function: Impaired renal function may affect gastrin metabolism, leading to false-positive test results. It's important to consider this factor when interpreting test outcomes, as false positives may result in unnecessary treatments or procedures.
In summary, Gastrin G17 is not only a pivotal regulator of gastrointestinal physiological activities but also a significant biomarker in clinical assessments of gastric health status and the auxiliary diagnosis of gastric disorders.
The performance data of the G17 Rapid Quantitative Test Kit(CLIA)
Manufacturer | SEKBIO |
Parameter | G17 |
Linearity | 1-50 pmol/L |
Detection limit | 1-7 pmol/L |
benchmark product | Biohit |
Test principle and procedure | G17: Add 50 μl of sample + 30 μl of Ra+ 30 μl of Rd incubation for 10 minutes. Wash with 200 μl of washing solution. Finally, add 100 μl of A + 100 μl of B. |
Six point calibration test results
Sample Concentration(pmol/L ) |
RLU | Average | CV | S/N | ||
0.00 | 336 | 317 | 272 | 308 | 10.66% | / |
0.95 | 7,079 | 6,915 | 6,708 | 6,901 | 2.69% | 22.38 |
7.89 | 51,814 | 52,710 | 52,495 | 52,340 | 0.89% | 169.75 |
15.97 | 109,076 | 102,804 | 100,351 | 104,077 | 4.32% | 337.55 |
33.01 | 212,540 | 213,548 | 207,825 | 211,304 | 1.45% | 685.31 |
56.27 | 352,165 | 342,591 | 372,118 | 355,625 | 4.24% | 1153.38 |
Precision
Sample | RLU | Concentration | Sample | RLU | Concentration |
8.00 | 50,141 | 7.54 |
33.00 | 223,548 | 34.98 |
52,700 | 7.93 | 211,104 | 32.99 | ||
53,957 | 8.13 | 218,921 | 34.24 | ||
53,773 | 8.10 | 221,541 | 34.66 | ||
53,963 | 8.13 | 218,329 | 34.15 | ||
53,458 | 8.05 | 225,533 | 35.30 | ||
54,073 | 8.15 | 204,392 | 31.91 | ||
51,764 | 7.79 | 218,887 | 34.24 | ||
54,222 | 8.17 | 213,227 | 33.33 | ||
54,555 | 8.22 | 199,373 | 31.11 | ||
AVE | 53,261 | 8.02 | AVE | 215,486 | 33.69 |
SD | 1367.30 | 0.21 | SD | 8439.72 | 1.35 |
CV | 2.57% | 2.64% | CV | 3.92% | 4.01% |
Limit of detection
Sample | RLU | Concentration | ||
Ag Diluent | 220 | 210 | 0.000 | 0.000 |
264 | 291 | 0.000 | 0.000 | |
192 | 261 | 0.000 | 0.000 | |
226 | 215 | 0.000 | 0.000 | |
217 | 246 | 0.000 | 0.000 | |
237 | 261 | 0.000 | 0.000 | |
227 | 236 | 0.000 | 0.000 | |
213 | 188 | 0.000 | 0.000 | |
218 | 245 | 0.000 | 0.000 | |
312 | 245 | 0.001 | 0.000 | |
AVE | 233 | 0.000 | ||
SD | 31 | 0.000 | ||
AVE+2*SD | 0.000 | |||
Linear
Sample( pmol/L) | Dilution ratio | Concentration value | Average | ||
56.31 | 1.000 | 57.08 | 55.37 | 60.68 | 56.31 |
28.15 | 0.500 | 27.68 | 24.87 | 26.42 | 26.65 |
14.08 | 0.250 | 13.65 | 13.42 | 13.42 | 13.79 |
7.04 | 0.125 | 6.76 | 6.76 | 6.80 | 6.91 |
3.52 | 0.063 | 3.37 | 3.48 | 3.47 | 3.48 |
1.71 | 0.031 | 1.69 | 1.75 | 1.68 | 1.71 |
Method Comparison
