FT3(Free Triiodothyronine)
The performance data of the FT3 Rapid Quantitative Test Kit(CLIA)
Manufacturer | SEKBIO |
Parameter | FT3 |
Linearity | 0.4-50 pmol/L |
Detection limit | 0.4 pmol/L |
benchmark product | Roche |
Test principle and procedure | FT3: Add 30 μl of sample + 30 μl of Rd, incubation for 10 minutes without cleaning, 30 μl of Ra+ 30 μl of Rc incubation for 5 minutes. Wash with 200 μl of washing solution. Finally, add 100 μl of A + 100 μl of B. |
Precision
Sample | RLU | Concentration | Sample | RLU | Concentration |
3.17 | 329,639 | 3.00 | 37.01 | 77,648 | 36.43 |
316,809 | 3.03 | 77,769 | 36.36 | ||
306,416 | 3.19 | 78,584 | 35.85 | ||
310,963 | 3.16 | 74,886 | 38.24 | ||
316,886 | 2.88 | 74,313 | 38.63 | ||
326,138 | 2.92 | 76,182 | 37.38 | ||
314,699 | 3.16 | 77,562 | 36.49 | ||
320,368 | 2.80 | 76,942 | 36.88 | ||
317,900 | 3.13 | 76,563 | 37.13 | ||
319,878 | 2.89 | 79,136 | 35.52 | ||
AVE | 317969.60 | 3.02 | AVE | 76958.50 | 36.89 |
SD | 6733.77 | 0.14 | SD | 1525.12 | 0.99 |
CV | 2.12% | 4.63% | CV | 1.98% | 2.68% |
Limit of detection
Sample | RLU | Concentration | ||
Ag Diluent | 2,154 | 2,156 | 0.03 | 0.03 |
1,986 | 1,978 | 0.02 | 0.02 | |
2,051 | 2,223 | 0.02 | 0.03 | |
2,208 | 2,064 | 0.03 | 0.02 | |
2,035 | 2,045 | 0.03 | 0.02 | |
2,124 | 2,062 | 0.02 | 0.02 | |
2,104 | 2,110 | 0.02 | 0.02 | |
2,065 | 2,056 | 0.02 | 0.02 | |
2,289 | 2,001 | 0.04 | 0.02 | |
2,110 | 2,096 | 0.02 | 0.02 | |
AVE | 2,096 | 0.02 | ||
SD | 80.22 | 0.01 | ||
AVE+2*SD | 0.04 | |||
Linear
Sample（ pmol/L) | Dilution ratio | Concentration value | Average | ||
67.14 | 1.0 | 66.72 | 67.09 | 67.60 | 67.14 |
54.33 | 0.8 | 56.51 | 53.26 | 57.89 | 55.89 |
41.52 | 0.6 | 45.28 | 44.44 | 43.68 | 44.46 |
28.72 | 0.4 | 31.81 | 32.73 | 31.77 | 32.10 |
15.91 | 0.2 | 16.53 | 17.96 | 17.05 | 17.18 |
3.10 | 0.0 | 3.05 | 3.13 | 3.12 | 3.10 |
Method Comparison
Reagents Stability
Reagent | Sample( pmol/L) | Average | Deviation |
2-8° | 50.00 | 135,719 | |
10.00 | 434,292 | ||
0.40 | 791,017 | ||
Antigen diluent | 841,178 | ||
random sample | 348,685 | ||
calf serum | 381,680 | ||
37℃ 1d | 50.00 | 134,405 | -0.97% |
10.00 | 424,298 | -2.30% | |
0.40 | 780,909 | -1.28% | |
Antigen diluent | 836,368 | -0.57% | |
random sample | 344,263 | -1.27% | |
calf serum | 373,148 | -2.24% | |
37℃ 4d | 50.00 | 127,883 | -5.77% |
10.00 | 392,923 | -9.53% | |
0.40 | 738,631 | -6.62% | |
Antigen diluent | 777,716 | -7.54% | |
random sample | 329,318 | -5.55% | |
calf serum | 346,663 | -9.17% | |
37℃ 7d | 50.00 | 121,498 | -10.48% |
10.00 | 371,456 | -14.47% | |
0.40 | 709,625 | -10.29% | |
Antigen diluent | 703,179 | -16.41% | |
random sample | 313,072 | -10.21% | |
calf serum | 337,341 | -11.62% |
Calibrator Stability
Reagent | Sample( pmol/L) | Average | Deviation |
2-8℃ | 66.99 | 43,902 | |
37.01 | 74,473 | ||
3.17 | 310,740 | ||
37℃ 1d | 66.99 | 43,667 | -0.53% |
37.01 | 76,993 | 3.38% | |
3.17 | 307,831 | -0.94% | |
37℃ 4d | 66.99 | 43,303 | -1.36% |
37.01 | 75,963 | 2.00% | |
3.17 | 306,706 | -1.30% | |
37℃ 7d | 66.99 | 44,214 | 0.71% |
37.01 | 72,454 | -2.71% | |
3.17 | 305,293 | -1.75% |
- Cardiac Markers
-
Tumor Marker
- CA50
-
CA125
- CA242
-
CA15-3
- CA19-9
- CA72-4
-
Pepsinogens I (PGI)
-
Human Epididymis 4 (HE4)
- Prostate-Specific Antigen (PSA)
- Squamous Cell Carcinoma (SCC)
- Neuron-Specific Enolase (NSE)
- Cytokeratin 19 Fragment (CYFRA21-1)
- Human Progastrin-releasing Peptide (ProGRP Tumor Marker)
- Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA II Tumor Marker)
- Alpha-fetoprotein(AFP)
- Inflammatory Marker
- Infectious Disease
- Hormones
- Thyroid Function
- Glucose Metabolism
- Bone Marker
- Others
-
Heterophilic Blocking Reagent
- Animal Diagnostics
