Email

Human Chitinase 3-like 1 ELISA Performance data

Introduction

Human Chitinase 3-like 1 (CHI3L1) is a protein that belongs to the chitinase protein family. It is also known by other names such as YKL-40 and HC-gp39. CHI3L1 is expressed in various tissues, including those related to the immune system, and its levels can be influenced by inflammatory processes.


Research suggests that CHI3L1 may play a role in various physiological and pathological conditions, including inflammation, tissue remodeling, and cancer. Elevated levels of CHI3L1 have been observed in conditions such as asthma, rheumatoid arthritis, and certain types of cancer. It is often considered a biomarker associated with inflammation and tissue damage.


Chitinase 3-like 1 (CHI3L1) has been studied for its clinical significance as a diagnostic biomarker in various diseases. Here are some examples of its relevance in different medical conditions:


Cancer: Elevated levels of CHI3L1 have been associated with certain types of cancer, including breast cancer, lung cancer, colorectal cancer, and ovarian cancer. In these cases, CHI3L1 may serve as a potential biomarker for cancer detection and prognosis. High levels of CHI3L1 are often correlated with more aggressive forms of cancer and poorer patient outcomes.


Inflammatory Diseases: CHI3L1 is often upregulated during inflammatory processes. Conditions such as rheumatoid arthritis, asthma, and inflammatory bowel disease may show increased levels of CHI3L1. Monitoring CHI3L1 levels in these diseases can provide insights into the severity of inflammation and may be useful in diagnosing and managing these conditions.


Elevated serum CHI3L1 levels have also been observed in some conditions characterized by inflammation and tissue remodeling, such as arthritis, severe bacterial infection, inflammatory bowel disease, and liver cirrhosis. In asthma, serum CHI3L1 levels have been reported to correlate positively with the severity of the disease and adversely with lung function


 Chitinase_comparison.png


Its ELISA protocol

Step 1: Add 100 μl diluted sample per well, and incubate at 37°C for 30 minutes.

Step 2: Wash three times with washing solution, 300 μl per well

Step 3: Incubate with antibody-HRP 100 μl per well, at 37°C for 30 minutes.

Step 4: Wash three times with washing solution, 300 μl per well 

Step 5: Mix the chromogenic solution A/B in a 1:1 ratio, add 100 μl per well, incubate at 37°C for 15 minutes, and add 50 μl per well of stop solution.

Step 6: Measure at an OD450 wavelength.


Calibration Curve

Calibrator

ng/ml

OD

OD

AVE

Cal 1

0

0.014

0.01

0.012

Cal 2

37.5

0.327

0.318

0.323

Cal 3

75

0.609

0.588

0.599

Cal 4

150

1.057

1.029

1.043

Cal 5

300

1.746

1.627

1.687

Cal 6

600

2.406

2.373

2.390 


Performance Data

1. Linearity data

 

Performance-Data-1.jpg

 

Linearity

OD

OD

OD

OD AVE

CV

Cal. 1

0.283

0.272

0.297

0.284

4.4%

Cal. 2

0.976

1.001

1.045

1.007

3.5%

Cal. 3

1.489

1.483

1.517

1.496

1.2%

Cal. 4

1.806

1.842

1.91

1.853

2.9%

Cal. 5

2.097

2.125

2.087

2.103

0.9%

Cal. 6

2.341

2.32

2.335

2.332

0.5%

Theoretical Conc.

Conc. ng/ml

Conc. ng/ml

Conc. ng/ml

 AVE ng/ml

CV

32.8

32.7

31.3

34.4

32.8

4.8%

139.4

136.4

141.0

149.1

142.2

4.5%

245.9

246.6

245.1

253.8

248.5

1.9%

352.5

338.9

351.0

375.0

355.0

5.2%

459.0

449.4

461.8

445.1

452.1

1.9%

565.6

570.6

558.9

567.3

565.6

1.1%


2. Repeatability data

 

Control

OD

OD

AVE

CV

Conc. ng/ml

Conc. ng/ml

AVE

CV

R1

0.582

0.624

0.597

6.1%

72.9

79.0

75.141

7.1%

0.563

0.596

70.1

74.9

0.57

0.626

71.1

79.3

0.573

0.678

71.6

87.1

0.565

0.597

70.4

75.0

R2

1.726

1.727

1.669

4.0%

313.3

313.6

296.372

6.8%

1.613

1.653

279.9

291.4

1.643

1.593

288.5

274.3

1.668

1.807

295.8

339.2

1.6

1.664

276.3

291.5

 

 

3. Method Comparison

 

Performance-Data-2.jpg


Data


Bench Mark

Sekbio


Bench Mark

Sekbio

Clinic Sample

OD value

Conc. ng/ml

OD value

Conc. ng/ml

Clinic Sample

OD value

Conc. ng/ml

OD value

Conc. ng/ml

S1

0.111

18.86

0.195

17.85

S24

0.285

57.39

0.502

49.73

S2

0.121

21.60

0.204

18.86

S25

0.496

95.42

0.757

75.38

S3

0.167

32.93

0.308

30.04

S26

0.539

102.81

0.836

83.49

S4

0.119

21.07

0.265

25.51

S27

0.622

116.94

1.059

107.14

S5

0.260

52.53

0.441

43.61

S28

0.385

75.92

0.604

59.94

S6

0.147

28.21

0.274

26.47

S29

0.191

38.29

0.380

37.44

S7

0.139

26.25

0.289

28.05

S30

0.268

54.10

0.385

37.95

S8

0.223

45.07

0.367

36.11

S31

1.124

203.25

1.609

173.18

S9

0.573

108.62

0.821

81.94

S32

0.250

50.54

0.521

51.63

S10

0.200

40.23

0.304

29.62

S33

0.305

61.20

0.518

51.33

S11

0.143

27.24

0.214

19.97

S34

1.655

306.80

2.477

320.44

S12

0.118

20.80

0.162

14.03

S35

0.302

60.64

0.505

50.03

S13

0.146

27.97

0.256

24.55

S36

0.386

76.10

0.528

52.33

S14

0.227

45.89

0.460

45.52

S37

0.321

64.21

0.452

44.71

S15

0.098

15.02

0.171

15.09

S38

0.342

68.10

0.567

56.23

S16

0.172

34.07

0.330

32.32

S39

0.575

108.96

0.913

91.51

S17

0.129

23.71

0.244

23.26

S40

0.246

49.74

0.476

47.12

S18

0.123

22.14

0.207

19.20

S41

0.484

93.35

0.632

62.75

S19

0.603

113.71

0.958

96.27

S42

0.157

30.60

0.279

26.99

S20

0.181

36.09

0.352

34.58

S43

0.365

72.31

0.728

72.43

S21

0.124

22.40

0.211

19.64

S44

0.983

177.88

1.532

162.81

S22

0.385

75.92

0.565

56.03

S45

0.693

128.94

1.157

117.99

S23

1.314

238.17

2.196

264.33

S46

0.739

136.71

1.051

106.27

 


Products