Manufacturer | SEKBIO |
Parameter | AMH |
Linearity | 0.01-23ng/ml |
Detection limit | 0.01ng/ml |
benchmark product | Roche |
Test principle and procedure | AMH: Add 30 μl of sample + 30 μl of Ra +30 μl of Rd and incubation for 10 minutes. Wash with 200 μl of washing solution. Finally, add 100 μl of A + 100 μl of B. |
Sample Concentration(ng/ml) |
RLU | Average | CV | S/N | ||
25.52 | 1,451,207 | 1,509,238 | 1,470,757 | 1,477,067 | 2.79% | 6249.93 |
12.82 | 760,735 | 770,216 | 757,399 | 762,783 | 0.89% | 3227.57 |
6.25 | 373,341 | 365,537 | 388,423 | 375,767 | 1.42% | 1589.99 |
2.34 | 144,041 | 137,127 | 139,993 | 140,387 | 3.49% | 594.02 |
0.12 | 7,352 | 7,608 | 6,823 | 7,261 | 2.65% | 30.72 |
0.00 | 232 | 242 | 235 | 236 | 3.01% | 1.00 |
Sample | RLU | Concentration | Sample | RLU | Concentration |
0.1 | 6,905 | 0.11 |
6.25 | 382,945 | 6.37 |
6,952 | 0.12 | 395,880 | 6.59 | ||
6,918 | 0.11 | 402,566 | 6.70 | ||
6,960 | 0.12 | 386,642 | 6.43 | ||
6,917 | 0.11 | 380,176 | 6.33 | ||
6,898 | 0.11 | 380,164 | 6.33 | ||
7,012 | 0.12 | 392,422 | 6.53 | ||
7,183 | 0.12 | 396,679 | 6.60 | ||
6,816 | 0.11 | 391,700 | 6.52 | ||
7,335 | 0.12 | 390,734 | 6.50 | ||
AVE | 6,990 | 0.12 | AVE | 389,991 | 6.490 |
SD | 154.77 | 0.00 | SD | 7458.69 | 0.12 |
CV | 2.21% | 2.27% | CV | 1.91% | 1.92% |
Sample | RLU | Concentration | ||
Ag Diluent | 227 | 232 | 0 | 0 |
237 | 224 | 0 | 0 | |
198 | 254 | 0 | 0.001 | |
223 | 255 | 0 | 0.001 | |
223 | 211 | 0 | 0 | |
224 | 200 | 0 | 0 | |
204 | 255 | 0 | 0.001 | |
227 | 222 | 0 | 0 | |
203 | 237 | 0 | 0.001 | |
211 | 221 | 0 | 0 | |
AVE | 224.40 | 0.000 | ||
SD | 17.30 | 0.00 | ||
AVE+2*SD | 0.001 |
Sample(ng/ml) | Dilution ratio | Concentration value | Average | ||
25.58 | 1.00 | 25.31 | 26.08 | 25.34 | 25.58 |
20.47 | 0.80 | 20.40 | 22.79 | 21.84 | 21.68 |
15.35 | 0.60 | 14.38 | 14.42 | 14.60 | 14.47 |
10.24 | 0.40 | 10.84 | 10.85 | 10.56 | 10.75 |
5.13 | 0.20 | 5.32 | 5.52 | 5.85 | 5.56 |
0.01 | 0.00 | 0.01 | 0.01 | 0.01 | 0.01 |