Testosterone (Testo)
Testosterone is the primary male sex hormone and an anabolic steroid. In male humans, testosterone plays a vital role in developing male reproductive tissues such as the testes and prostate and promoting secondary sexual characteristics such as increased muscle and bone mass and the growth of body hair.
The increased Testo level may be caused by: tumor, hermaphroditism, polycystic ovary syndrome, etc.
The decreased level reflects testis hypoplasia, hypophysis of the anterior lobe, cancer, etc. Combined with serum LH and FSH test results, it can identify primary or secondary testicular dysfunction and precocious puberty in adolescent children.
The performance data of the Testosterone (TES) Rapid Quantitative Test Kit (FIA)
Manufacturer | SEKBIO |
Parameter | Testosterone (TES) Rapid Quantitative Test Kit (FIA) |
Linearity | 0.2~15ng/ml |
Detection limit | 0.2ng/mL |
Benchmark product | Roche |
1 Limit of detection
Result(C/T) | Concentration | |
0 | 0.18 | |
1 | 0.64100 | 0.96675 |
2 | 0.79513 | 0.91924 |
3 | 0.73527 | 1.06822 |
4 | 0.77956 | 0.86043 |
5 | 0.75007 | 1.10303 |
6 | 0.63345 | 0.94187 |
7 | 0.78466 | 0.84868 |
8 | 0.69630 | 0.95063 |
9 | 0.71701 | 1.08039 |
10 | 0.70040 | 1.02653 |
11 | 0.76801 | 1.11575 |
12 | 0.64208 | 0.89674 |
13 | 0.79987 | 1.05212 |
14 | 0.68063 | 0.91094 |
15 | 0.72378 | 0.82531 |
16 | 0.67849 | 0.96335 |
17 | 0.66497 | 0.90646 |
18 | 0.75392 | 1.03400 |
19 | 0.78819 | 0.91084 |
20 | 0.65704 | 0.88530 |
AVE | 0.71949 | 0.96333 |
SD | 0.05621 | / |
AVE+2*SD | 0.83191 | / |
regression equation | y=1.35465x+0.71949 | |
LoB(ng/mL) | 0.08299 |
2 Linear
Cal | Concentration | Result | AVERAGE |
S1 | 0.18 | 0.19 | 0.18 |
0.17 | |||
0.17 | |||
S2 | 0.52 | 0.48 | 0.53 |
0.57 | |||
0.54 | |||
S3 | 1.36 | 1.44 | 1.35 |
1.34 | |||
1.27 | |||
S4 | 4.46 | 4.17 | 4.47 |
4.98 | |||
4.25 | |||
S5 | 10.03 | 9.98 | 10.25 |
10.53 | |||
10.25 | |||
S6 | 16.04 | 15.45 | 15.36 |
15.30 | |||
15.34 |
3 Accuracy
Cal | concentration | Result | Bias |
A1 | 0.52 | 0.51 | -1.3% |
0.48 | -8.7% | ||
0.47 | -9.8% | ||
A2 | 1.36 | 1.47 | 7.8% |
1.31 | -3.5% | ||
1.37 | 0.9% | ||
A3 | 10.03 | 9.68 | -3.5% |
10.84 | 8.1% | ||
10.18 | 1.5% |
4 Precision
Cal | Concentration | Cal | Concentration | Cal | Concentration |
R1 | 0.52 | R2 | 1.56 | R3 | 8.62 |
0.51 | 1.52 | 8.94 | |||
0.46 | 1.27 | 9.19 | |||
0.45 | 1.55 | 9.11 | |||
0.50 | 1.21 | 9.81 | |||
0.59 | 1.23 | 9.00 | |||
0.53 | 1.50 | 11.24 | |||
0.59 | 1.41 | 10.81 | |||
0.53 | 1.21 | 9.43 | |||
0.56 | 1.38 | 10.45 | |||
AVE | 0.52 | AVE | 1.38 | AVE | 9.66 |
SD | 0.05 | SD | 0.14 | SD | 0.89 |
CV | 8.9% | CV | 10.4% | CV | 9.2% |
5 Method Comparison
Sample | Roche | Sekbio | Sample | Roche | Sekbio |
1 | 6.17 | 5.14 | 31 | 4.20 | 4.08 |
2 | 10.37 | 11.04 | 32 | 3.45 | 3.55 |
3 | 4.30 | 4.16 | 33 | 0.77 | 0.95 |
4 | 11.19 | 11.38 | 34 | 13.24 | 12.21 |
5 | 0.83 | 0.79 | 35 | 6.56 | 6.66 |
6 | 0.81 | 0.78 | 36 | 0.31 | 0.30 |
7 | 0.42 | 0.43 | 37 | 1.88 | 2.00 |
8 | 1.19 | 1.17 | 38 | 0.79 | 0.96 |
9 | 2.51 | 2.30 | 39 | 1.20 | 1.07 |
10 | 2.93 | 2.62 | 40 | 0.90 | 0.78 |
11 | 0.92 | 0.91 | 41 | 2.14 | 2.24 |
12 | 1.38 | 1.27 | 42 | 9.33 | 8.11 |
13 | 2.88 | 3.02 | 43 | 0.44 | 0.45 |
14 | 0.31 | 0.28 | 44 | 9.91 | 10.73 |
15 | 10.06 | 8.67 | 45 | 10.18 | 8.86 |
16 | 10.50 | 8.48 | 46 | 0.95 | 0.90 |
17 | 11.04 | 9.99 | 47 | 0.98 | 1.06 |
18 | 5.41 | 6.14 | 48 | 6.35 | 6.80 |
19 | 3.01 | 3.08 | 49 | 1.42 | 1.64 |
20 | 0.22 | 0.25 | 50 | 0.51 | 0.55 |
21 | 3.18 | 3.96 | 51 | 0.53 | 0.56 |
22 | 8.94 | 7.79 | 52 | 8.45 | 9.89 |
23 | 2.45 | 2.88 | 53 | 1.31 | 1.19 |
24 | 1.32 | 1.26 | 54 | 1.45 | 1.32 |
25 | 0.20 | 0.20 | 55 | 10.58 | 11.31 |
26 | 4.71 | 4.87 | 56 | 2.47 | 2.42 |
27 | 2.24 | 2.25 | 57 | 0.35 | 0.32 |
28 | 8.56 | 7.70 | 58 | 1.01 | 1.10 |
29 | 0.20 | 0.23 | 59 | 0.25 | 0.21 |
30 | 2.41 | 2.24 | 60 | 7.56 | 7.66 |
Testosterone (Testo) Products
Antibody | Application |
Sheep Anti-human Testo mAb | For immunodiagnostic: ELISA, LFA, CLIA |
Testosterone Intro
Testosterone, with the molecular formula C19H28O2, is a steroid hormone secreted by the testes of men or the ovaries of women. It has the functions of maintaining muscle strength and mass, maintaining bone density and strength, refreshing, and improving physical fitness. The adrenal glands also secrete a small amount of testosterone.
Testosterone is a secreted glycoprotein initially identified from the enrichment medium of mouse Sertoli cells. A small molecular weight cytoskeleton-associated protein binds tightly to the cell surface through receptor-associated proteins.