SEKBIO Heterophilic Blocking Reagent (HBR Product) Detail

Heterophilic antibodies can impact a variety of assays, but they have the most significant effect on sandwich immunoassays.

The traditional passive blocking method involves using non-specific substances (such as mouse IgG, mouse serum, non-specific monoclonal antibodies, polymeric IgG, etc.) to block the binding of human heterophilic antibodies. The blocking effect of all methods depends on the avidity of human heterophilic antibodies.

Our HBR products achieves blocking in a completely different way. HBR can actively bind to human heterophilic antibodies, resulting in a steric hindrance effect and preventing interference. Our HBR products offers the following advantages over traditional blockers:

Save your cost! Only a small amount of HBR is required for blocking without reducing the detection signal.

Our HBR can block all subtypes of anti-mouse monoclonal antibodies (such as anti-mouse IgG1, anti-mouse IgG2, etc.).

Our HBRcan be applicable for all immunoassay and is not limited to certain assays.


Storage temperature:

Store at -20°C or below.

Instructions for use:

Thaw the blocking reagent and add it to the reagent components, mix well.

Comparison Data of Blocker Between SEKBIO's with Top Brands'


This evaluation experiment utilized a fluorescent lateral flow assay and tested six clinical samples. The results demonstrated that SEKBIO's blocker outperformed Top brand’s blocker.