1. What Are Cardiac Biomarkers?

Cardiac biomarkers are proteins, enzymes and other molecules released into the bloodstream when cardiac tissue is damaged or under stress. In IVD diagnostics, they serve as measurable indicators for conditions including acute myocardial infarction (AMI), heart failure, preeclampsia and inflammatory states that affect cardiovascular function.

The most clinically important cardiac biomarkers in point-of-care and laboratory testing are cardiac Troponin I (cTnI) and Troponin T — the gold standard for AMI diagnosis — along with D-Dimer for thrombosis risk, CK-MB for early reinfarction detection, BNP/NT-proBNP for heart failure staging, and CRP and PCT for cardiac inflammation and sepsis stratification.

For IVD manufacturers, the antibody pair — a matched capture and detection antibody set — is the most critical reagent component. Its epitope specificity, cross-reactivity profile, and platform compatibility directly determine assay sensitivity, specificity and lot-to-lot reproducibility. Sekbio supplies pre-validated, production-grade antibody pairs across all major cardiac biomarker targets.

One Validation. Consistent Performance. Every Batch.

Sekbio cardiac antibodies are manufactured at industrial scale with tight lot-to-lot CV and full ISO 13485-certified traceability. Validate once, produce with confidence.

2. Cardiac Biomarker Antibody Products

All products are available as matched antibody pairs (capture + detection), individual clones or colloidal gold conjugates. Click any product for full specifications.

Biomarker Clinical Use Formats Key Specs Details
Cardiac Troponin I (cTnI) AMI diagnosis, rule-in/rule-out CLIALFAFIA LoD <0.01 ng/mL; no skeletal TnI cross-reaction View →
D-Dimer DVT, PE exclusion, coagulation LFACLIAELISA Paired mAbs; fibrin-specific; low interference View →
CK-MB Early AMI, re-infarction detection LFACLIAELISA No CK-MM or CK-BB cross-reactivity View →
Procalcitonin (PCT) Sepsis diagnosis, antibiotic stewardship LFACLIAFIA Detects intact PCT; LoD <0.05 ng/mL View →
C-Reactive Protein (CRP) Inflammation, cardiovascular risk LFACLIAELISA Wide dynamic range; high-sensitivity CRP available View →
H-FABP Early AMI (<3 h onset), rule-out LFAELISA Rises within 1–3 h of injury; cardiac-specific View →
Troponin T AMI, chronic heart disease monitoring CLIAELISA Cardiac-specific isoform; complementary to cTnI View →
PlGF / sFlt-1 Preeclampsia prediction and monitoring CLIAELISA Paired PlGF + sFlt-1 ratio assay components View →
Erythropoietin (EPO) Anemia, CKD, cardiac load assessment CLIAELISA High sensitivity; broad linear range View →
Ferritin Iron deficiency, cardiac inflammation LFACLIAELISA Stable across wide concentration range; low HOOK effect View →
Myoglobin (MYO) Early AMI rule-out; rises within 1–4 h of onset LFACLIAELISA GSA501 (capture) + GSA503 (detection); validated vs Roche ECLIA; earliest-rising cardiac marker View →
NT-proBNP Heart failure diagnosis, NYHA staging and monitoring LFACLIAELISA 7 mouse mAbs + humanized variants; epitopes 13–76; HAMA-resistant humanized pair available View →
View Full Antibody Catalog →
10+
Cardiac Biomarker Targets
<10%
Lot-to-Lot CV
30+
Countries Served
ISO
13485 Certified

3. Assay Formats Supported

Sekbio cardiac antibody pairs are pre-validated and optimized for four IVD assay platforms. Each pair ships with platform-specific recommended working concentrations.

Lateral Flow Assay (LFA)

Colloidal gold and latex bead conjugates for rapid, instrument-free POC testing. Suitable for cTnI, D-Dimer, CRP, CK-MB, H-FABP and Ferritin.

CLIA

Chemiluminescent immunoassay pairs for automated analyzer platforms. High-sensitivity formats available for cTnI, PCT and D-Dimer.

ELISA

Sandwich ELISA-grade pairs for laboratory quantification. Biotin/streptavidin and HRP-conjugated detection antibodies available.

FIA (Fluorescence)

Fluorescent microsphere and time-resolved fluorescence conjugates for quantitative POC analyzers. Validated for cTnI and PCT.

Not sure which format is right for your assay? Sekbio's technical team can advise on antibody pair selection, conjugation chemistry and assay configuration based on your target LoD, matrix and instrument platform. Contact us or explore our antibody development services for custom pair screening.

4. Why IVD Manufacturers Choose Sekbio

The most common bottleneck in cardiac IVD development is antibody qualification — testing dozens of clone combinations across multiple platforms before finding a pair that meets sensitivity and specificity targets. Sekbio eliminates this step.

Pre-Validated Pairs

Every cardiac antibody pair is screened in a real sandwich immunoassay before catalog listing. You receive a pair that works on your target platform from day one.

Industrial Scale, IVD Grade

Antibodies are produced in CHO or hybridoma bioreactors at batch sizes from 100 mg to gram scale, with full ISO 13485 documentation for regulatory submissions.

Tight Lot-to-Lot Consistency

Lot-to-lot CV <10% on key performance parameters means your assay QC stays in control across production batches — no repeat validation cycles.

Technical Support

Our antibody scientists provide hands-on support for coating optimization, conjugation troubleshooting and cut-off calibration — included with every order.

5. Frequently Asked Questions — Cardiac Biomarker Antibodies

What are cardiac biomarker antibody pairs?

Cardiac biomarker antibody pairs are matched sets of capture and detection antibodies used in sandwich immunoassays to quantify heart-related proteins in blood samples. They are the core reagent components in CLIA, lateral flow assay (LFA), ELISA and FIA diagnostic tests for conditions such as acute myocardial infarction, heart failure and preeclampsia.

Which cardiac biomarkers does Sekbio supply antibody pairs for?

Sekbio supplies antibody pairs for ten cardiac targets: Cardiac Troponin I (cTnI), D-Dimer, CK-MB, Procalcitonin (PCT), C-Reactive Protein (CRP), Heart-type Fatty Acid Binding Protein (H-FABP), Troponin T, PlGF/sFlt-1, Erythropoietin (EPO) and Ferritin. All pairs are pre-validated on at least one clinical assay platform before catalog release.

Are Sekbio cardiac antibody pairs pre-validated on LFA and CLIA platforms?

Yes. Every antibody pair in the Sekbio cardiac portfolio is pre-validated on the platform it is intended for — LFA, CLIA, ELISA or FIA — before being released to customers. Platform-specific data including signal-to-noise ratios, working concentration ranges and lot-to-lot CV values are available upon request alongside the product datasheet.

What is the minimum order quantity (MOQ) for cardiac antibody pairs?

The standard MOQ is 1 mg per antibody clone, which is sufficient for initial assay feasibility studies. For scale-up and routine production, quantities from 10 mg to gram scale are available with full ISO 13485-certified batch documentation and Certificate of Analysis.

How does Sekbio ensure batch-to-batch consistency for cardiac antibodies?

Each batch undergoes SEC-HPLC purity testing, SDS-PAGE, binding activity ELISA and platform-specific functional assays. Lot-to-lot CV for key performance parameters is maintained below 10%. All manufacturing is conducted under an ISO 13485-certified quality management system, and full traceability documentation is provided with every shipment.

Can Sekbio develop custom cardiac biomarker antibody pairs not listed in the catalog?

Yes. If you require an antibody pair for a cardiac target not in our catalog, Sekbio offers end-to-end custom antibody development — from antigen design and immunization to hybridoma screening, pairing validation and stable cell line establishment. Delivery for a complete custom project is typically 8–12 weeks. Explore our antibody development platforms for details on the workflow and timelines.

What documentation is provided with cardiac antibody shipments?

Every shipment includes a Certificate of Analysis (CoA) with purity, activity and lot number; a Safety Data Sheet (SDS); and, upon request, full raw QC data including SEC-HPLC chromatograms and functional assay curves. All documentation is aligned with ISO 13485 traceability requirements for IVD regulatory submissions.

Ready to Build Your Cardiac Assay?

Request a sample, download datasheets, or speak with a Sekbio antibody scientist about your assay requirements.

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