Heart-Type Fatty Acid Binding Protein Antibody for Early Cardiac AMI Diagnosis & POCT Development
Heart-type Fatty Acid Binding Protein (H-FABP), also designated FABP3, is a 15 kDa cytoplasmic protein expressed at high concentration in cardiomyocytes and, to a lesser degree, in skeletal muscle and brain tissue. H-FABP's primary function is intracellular long-chain fatty acid transport — carrying fatty acids from the sarcolemma to mitochondria for beta-oxidation, which provides ~70% of myocardial energy. H-FABP constitutes approximately 5% of total cytoplasmic protein in cardiomyocytes, making it one of the most abundant myocardial proteins.
For IVD assay development, H-FABP is clinically significant as the earliest cardiac biomarker for acute myocardial infarction (AMI). Due to its small size (15 kDa vs 24 kDa for Myoglobin, 23 kDa for cTnI), H-FABP enters the bloodstream within 30 minutes to 3 hours of myocardial injury onset — achieving peak serum concentration at 4–8 hours and returning to baseline within 24 hours. This early-rise, early-washout profile complements cTnI (which rises later but remains elevated for 7–14 days), making H-FABP particularly useful for:
Sekbio supplies a complete H-FABP antibody panel — mouse capture (S01-FABP-1M), humanized detection (S01-FABP-2H), mouse detection (S01-FABP-2M), and recombinant H-FABP antigen (S01-FABP-1) — validated for CLIA and LFA formats.
Mouse and humanized detection antibodies plus recombinant antigen for complete H-FABP immunoassay development.
| Catalog No. | Product Name | Format / Species | Purity | Intended Use | Storage |
|---|---|---|---|---|---|
| S01-FABP-1M | Mouse Anti-H-FABP/FABP3 mAb (Capture) | Mouse IgG | >90% SEC-HPLC | Capture — CLIA, LFA | +2°C to +8°C |
| S01-FABP-2H | Humanized Anti-H-FABP/FABP3 mAb (Detection) | Humanized IgG | >90% SEC-HPLC | Detection — CLIA, LFA | +2°C to +8°C |
| S01-FABP-2M | Mouse Anti-H-FABP/FABP3 mAb (Detection) | Mouse IgG | >90% SEC-HPLC | Detection — CLIA, LFA | +2°C to +8°C |
| S01-FABP-1 | Recombinant H-FABP/FABP3 Antigen | Recombinant (E. coli) | >80% SDS-PAGE | Calibrator / Positive Control | −20°C ±2°C |
MOQ 1 mg. OEM quantities (10 mg–gram scale) available. Contact info@sekbio.com for bulk pricing and current lot availability.
Designed for the speed and sensitivity required in emergency AMI diagnosis and point-of-care cardiac biomarker panel development.
H-FABP is detectable in serum as early as 30 minutes after myocardial injury — 1–2 hours before cTnI elevation and 2–3 hours before cTnT in conventional assays. This early window is critical for AMI rule-in in emergency triage, enabling earlier treatment decisions and better patient outcomes in the 0–3 hour presentation window.
S01-FABP-2H is a humanized anti-H-FABP antibody combining a human IgG constant domain with mouse variable regions. The humanized format reduces anti-mouse background in assay formats and is preferred for platforms using human Fc-based secondary detection or when assay specificity for cardiac H-FABP over skeletal muscle FABP is critical.
All H-FABP antibodies are validated for both quantitative CLIA analyzer formats and lateral flow POCT rapid test formats. H-FABP POCT lateral flow assays provide semi-quantitative or quantitative H-FABP results within 10–15 minutes — ideal for emergency department triage and pre-hospital AMI assessment.
S01-FABP-1 recombinant H-FABP antigen (E. coli expression) provides a defined calibrator material for assay development and kit quality control. Using a recombinant antigen ensures consistent and reproducible calibration curve preparation independent of variable native H-FABP purification yield.
H-FABP is designed for use in multi-marker cardiac panels alongside cTnI and CK-MB. Sekbio supplies all three cardiac biomarker antibody pairs — enabling a single-supplier solution for complete cardiac emergency panel raw material. Contact info@sekbio.com for combined cardiac panel quotes and technical guidance.
ISO 13485-compliant manufacturing in Shenzhen, China. MOQ 1 mg for R&D; gram-scale production supply for OEM manufacturing. Supports CE, NMPA, and FDA regulatory submissions. Three-year shelf life from manufacturing date with full COA provided per lot.
H-FABP antibody panel for cardiac emergency diagnostics, AMI rule-in, and POCT kit development.
In patients presenting with chest pain, the 0–3 hour window is critical: cTnI and cTnT may be negative within the first 1–2 hours of symptom onset, creating a diagnostic gap. H-FABP rises within 30–60 minutes of AMI onset and achieves positive detection at 3 hours in >90% of confirmed AMI cases. H-FABP POCT assays using Sekbio antibodies support early AMI rule-in during this critical window, enabling faster treatment decisions and reduced time to reperfusion therapy.
Following an initial AMI, cardiac troponin remains elevated for 7–14 days, making it unreliable for detecting re-infarction. H-FABP's 24-hour washout kinetics restore baseline levels rapidly, making a new H-FABP elevation after baseline normalisation a sensitive indicator of recurrent myocardial injury. Serial H-FABP measurement in the 24 hours after primary AMI treatment supports re-infarction surveillance in high-risk patients in coronary care units.
The three-marker cardiac panel (H-FABP + cTnI + CK-MB) provides superior diagnostic performance over any single marker for early ACS assessment. H-FABP provides sensitivity in the 0–3 hour window; cTnI provides high specificity from 3–24 hours; CK-MB enables re-infarction detection. Sekbio supplies all three antibody pairs for complete cardiac panel lateral flow strip or CLIA analyzer kit development — from a single ISO 13485 certified supplier.
H-FABP POCT lateral flow rapid tests are high-demand IVD products in emergency medicine, ambulance services, and primary care. Quantitative fluorescent H-FABP POCT assays using Sekbio S01-FABP-1M and S01-FABP-2H/2M antibodies deliver results in <15 minutes from whole blood or serum. OEM rapid test manufacturers can integrate Sekbio's antibody panel with ISO 13485-compliant batch data for CE and NMPA submissions. Visit our Products page for related cardiac biomarkers, or contact our team.
Technical and commercial questions from IVD R&D engineers and procurement teams.
H-FABP (Heart-type Fatty Acid Binding Protein, FABP3) is a 15 kDa cytoplasmic protein that constitutes ~5% of cardiomyocyte cytoplasmic protein. Its small size enables rapid diffusion into circulation — rising within 30 minutes to 3 hours of AMI onset, 1–2 hours before cTnI. This early detection window is H-FABP's primary clinical advantage: it enables AMI rule-in when cTnI is still negative, improving diagnostic accuracy in the critical first 3 hours after chest pain onset.
Sekbio supplies: S01-FABP-1M (mouse capture antibody, purity >90% SEC-HPLC), S01-FABP-2H (humanized detection antibody), S01-FABP-2M (mouse detection antibody), and S01-FABP-1 (recombinant H-FABP antigen for calibrators). All available from MOQ 1 mg with Certificate of Analysis.
All H-FABP antibodies are validated for CLIA (quantitative chemiluminescence immunoassay) and LFA (lateral flow assay/POCT rapid test) sandwich formats. H-FABP POCT is primarily developed for emergency department triage with result turnaround <15 minutes from whole blood or serum.
The commonly used clinical H-FABP cutoff for AMI is >6 ng/mL in serum at presentation. At this threshold, H-FABP provides sensitivity of 80–95% within the first 3 hours of AMI onset, superior to cTnI in this early window. H-FABP returns to baseline within 24 hours, enabling re-infarction detection. Clinical sensitivity and specificity values depend on the specific assay format and population.
MOQ is 1 mg for R&D evaluation; OEM production quantities from 10 mg to gram scale available. S01-FABP-1M, -2H, -2M antibodies: +2°C to +8°C short-term; −20°C long-term. Avoid repeated freeze/thaw cycles. S01-FABP-1 antigen: −20°C ±2°C. Shelf life: 3 years from manufacturing date.
Yes. Sekbio supplies antibody pairs for H-FABP (S01-FABP series), cTnI, and CK-MB — enabling development of a complete three-marker cardiac emergency panel from one ISO 13485 certified supplier. Combined panel antibody packages are available for LFA strip and CLIA analyzer kit development. Contact info@sekbio.com for cardiac panel quotes, or visit our Platforms page for antibody development services.
Request the full technical datasheet, cardiac panel antibody specifications, or discuss OEM supply with our team.