Heart Failure Monoclonal Antibody Panel for CLIA & ELISA Immunoassay Development
NT-proBNP (N-terminal pro-brain natriuretic peptide) is the biologically inactive 76-amino acid N-terminal fragment released from proBNP-108 when cardiac ventricular myocytes are stretched under conditions of pressure or volume overload. The active hormone BNP (C-terminal fragment) and NT-proBNP are released in equimolar amounts, but NT-proBNP has a significantly longer half-life (60–120 minutes vs 20 minutes for BNP), conferring superior assay stability.
NT-proBNP is the primary biochemical marker for heart failure diagnosis, staging, and monitoring per ESC, ACC/AHA, and CCS clinical guidelines. The ESC recommends age-stratified diagnostic cut-offs: <50 years = 450 pg/mL; 50–75 years = 900 pg/mL; >75 years = 1,800 pg/mL for acute HF. Sekbio supplies one of the broadest NT-proBNP antibody panels available — 7 mouse mAbs covering epitopes 13–76 plus a humanized variant (S01-A1027H) and sheep polyclonal alternative (S01-A1026S).
The matched recombinant NT-proBNP antigen (S01-P1023) enables traceable calibrator preparation, assay validation, and positive control manufacture for CLIA and ELISA NT-proBNP kit development.
Seven mouse mAbs covering distinct NT-proBNP epitopes, plus humanized and sheep polyclonal variants, and matched recombinant antigen.
| Catalog No. | Product Name | Species / Format | Epitope (aa) | Purity | Platform | Storage |
|---|---|---|---|---|---|---|
| S01-A1021M | Mouse Anti-NT-proBNP mAb | Mouse mAb | 31–39 | >90% SEC-HPLC | CLIA / ELISA | +2°C to +8°C |
| S01-A1022M | Mouse Anti-NT-proBNP mAb | Mouse mAb | 13–24 | >90% SEC-HPLC | CLIA / ELISA | +2°C to +8°C |
| S01-A1023M | Mouse Anti-NT-proBNP mAb | Mouse mAb | 67–76 | >90% SEC-HPLC | CLIA / ELISA | +2°C to +8°C |
| S01-A1025M | Mouse Anti-NT-proBNP mAb | Mouse mAb | 27–31 | >90% SEC-HPLC | CLIA / ELISA | +2°C to +8°C |
| S01-A1026S | Sheep Anti-NT-proBNP pAb | Sheep Polyclonal | 42–46 | >85% SEC-HPLC | CLIA / ELISA | +2°C to +8°C |
| S01-A1027M | Mouse Anti-NT-proBNP mAb | Mouse mAb | 39–48 | >90% SEC-HPLC | CLIA / ELISA | +2°C to +8°C |
| S01-A1027H | Humanized Anti-NT-proBNP mAb | Humanized mAb | 39–48 | >90% SEC-HPLC | CLIA / ELISA | +2°C to +8°C |
| S01-P1023 | Recombinant NT-proBNP Antigen | Recombinant | Full-length | >80% SDS-PAGE | Calibrator / Control | −20°C ±2°C |
Recommended pairs: S01-A1021M (ep 31–39) + S01-A1023M (ep 67–76) for CLIA; S01-A1022M (ep 13–24) + S01-A1027H (ep 39–48, humanized) for HAMA-prone samples. MOQ 1 mg. Contact info@sekbio.com for pair selection guidance.
The broadest NT-proBNP antibody panel from a single ISO 13485-certified IVD raw material supplier.
Sekbio's NT-proBNP antibody panel covers seven non-overlapping epitopes spanning residues 13–76 of the 76-aa NT-proBNP sequence. This multi-epitope coverage enables systematic pair screening to identify the optimal capture/detection combination for your specific CLIA or ELISA platform sensitivity requirement.
S01-A1027H is the humanized counterpart to S01-A1027M — substituting human framework residues while preserving the murine CDRs targeting epitope 39–48. For CLIA analyzers, the humanized detection antibody significantly reduces false results caused by human anti-mouse antibodies (HAMA) present in rheumatoid factor-positive or biologic-treated patient samples.
S01-A1026S sheep polyclonal anti-NT-proBNP (epitope 42–46) provides a complementary non-rodent detection option for bridging assay designs where murine antibody interference is a concern. Polyclonal detection paired with a mouse mAb capture creates a heterologous format that further reduces HAMA hook effects in clinical serum matrices.
The NT-proBNP antibody pairs are validated to achieve the analytically challenging 1–35,000 pg/mL dynamic range required for clinical NT-proBNP assays — spanning the HF rule-out threshold (125 pg/mL), diagnostic cut-offs (450–1,800 pg/mL age-stratified), and high-concentration decompensated HF values (>5,000 pg/mL).
S01-P1023 recombinant NT-proBNP antigen enables in-house calibrator preparation traceable to international reference materials. Eliminating the variability of native NT-proBNP from human plasma pools, S01-P1023 provides a consistent, well-characterised reference material for IVD kit development and production QC.
All NT-proBNP antibodies are manufactured under ISO 13485 QMS with inter-batch CV <10%. Each lot is released with a Certificate of Analysis covering appearance, purity (SEC-HPLC), molecular weight, and functional binding activity in sandwich ELISA. OEM quantities from gram to 10 g scale available for production supply.
NT-proBNP antibody panel validated for heart failure diagnostics, CLIA analyzer development, and OEM kit supply.
NT-proBNP is the primary biochemical marker for HF diagnosis and NYHA functional class stratification. CLIA assays using Sekbio's antibody pair enable the age-stratified ESC diagnostic cut-offs (450/900/1,800 pg/mL). NT-proBNP <125 pg/mL effectively rules out chronic HF with high negative predictive value, reducing unnecessary echocardiography referrals.
In emergency department dyspnoea assessment, NT-proBNP >300 pg/mL rules in HF as the cause of acute dyspnoea with >90% NPV. CLIA analyzers using Sekbio's NT-proBNP antibody pair enable rapid (30-minute) quantitative results to differentiate cardiac from pulmonary causes and guide immediate diuretic therapy decisions.
Serial NT-proBNP measurements guide guideline-directed medical therapy (GDMT) titration in HFrEF. A >30% NT-proBNP reduction from baseline predicts clinical improvement. NT-proBNP is also independently prognostic in acute coronary syndromes, pulmonary embolism (risk stratification), and CKD (cardiovascular event prediction).
IVD manufacturers developing CE-marked or NMPA-registered NT-proBNP assays can build on Sekbio's multi-epitope antibody panel with ISO 13485-compliant batch documentation, lot-to-lot consistency data, and clinical correlation reports. The humanized S01-A1027H simplifies HAMA interference management in regulatory submissions for global market entry.
Technical and commercial questions from IVD R&D engineers and procurement teams.
NT-proBNP is the biologically inactive 76-aa N-terminal fragment released from proBNP-108 under cardiac ventricular stress. Its long half-life (60–120 min vs 20 min for BNP) provides greater assay stability. NT-proBNP is the primary heart failure biomarker per ESC, ACC/AHA, and CCS guidelines, with age-stratified diagnostic cut-offs of 450/900/1,800 pg/mL for acute HF.
Sekbio supplies: S01-A1021M (ep 31–39), S01-A1022M (ep 13–24), S01-A1023M (ep 67–76), S01-A1025M (ep 27–31), S01-A1026S (sheep, ep 42–46), S01-A1027M (ep 39–48), S01-A1027H (humanized, ep 39–48), and S01-P1023 (recombinant antigen). Seven distinct epitopes enable comprehensive pair screening.
Recommended starting pairs: S01-A1021M (capture, ep 31–39) + S01-A1023M (detection, ep 67–76) for standard CLIA; S01-A1022M (capture, ep 13–24) + S01-A1027H (detection, ep 39–48, humanized) for HAMA-sensitive applications. Technical support is available for custom pair optimization.
S01-A1027H reduces assay interference from human anti-mouse antibodies (HAMA) — present in approximately 40% of patients on biologic therapies or with autoimmune conditions. HAMA can cause false elevations or false negatives in NT-proBNP CLIA assays. The humanized variant targets the identical epitope 39–48 but with human IgG framework, minimising HAMA binding.
MOQ is 1 mg for R&D evaluation. OEM production quantities from 10 mg to gram scale are available. All mouse mAbs and humanized antibodies: +2°C to +8°C short-term; −20°C long-term. S01-A1026S sheep polyclonal: same conditions. S01-P1023 antigen: −20°C ±2°C. Shelf life 3 years.
Yes. Sekbio provides clinical comparison data for selected NT-proBNP antibody pairs benchmarked against the Roche Elecsys proBNP II assay. Correlation reports are available upon request and support CE, NMPA, and FDA 510(k) regulatory submission packages. Contact info@sekbio.com for the full technical datasheet or visit our Cardiac Biomarkers page.
Request the full technical datasheet, pair selection guidance, or discuss OEM supply with our team.