Broad-Spectrum Sulfonamide Group Antibody & Trimethoprim (TMP) Antibody for Food Safety ELISA & LFA IVD Development
Sulfonamide residue antibodies are monoclonal antibodies raised against sulfonamide drug haptens conjugated to a carrier protein such as BSA. Because sulfonamides are small synthetic antibacterial molecules, they are detected using competitive inhibition immunoassay formats: free sulfonamide residue in a food sample competes with hapten-protein conjugate for a fixed amount of antibody, so higher residue concentrations produce weaker assay signal. This competitive format is the basis for both quantitative ELISA kits and colloidal-gold lateral flow (LFA) rapid test strips used throughout the food safety supply chain, from farm-gate screening to import border control testing.
Sulfonamides are a large class of synthetic antibacterial drugs widely used in veterinary medicine across livestock, poultry, and aquaculture for treating and preventing bacterial infections. Unlike banned compounds such as chloramphenicol, sulfonamides are not prohibited — they are regulated with maximum residue limits (MRLs) in most jurisdictions, reflecting their legitimate, high-volume veterinary use. Precisely because of that high usage volume, sulfonamides are among the most frequently detected antibiotic residue classes in food safety monitoring programs globally, making broad-spectrum group screening — rather than compound-by-compound testing — the practical standard for routine surveillance. Trimethoprim (TMP), a synergist commonly co-administered with sulfonamides (as in sulfamethoxazole-trimethoprim combination products) to enhance antibacterial activity, is tested alongside the sulfonamide class for the same reason: the two drug types are so often used together that residue monitoring programs treat them as a linked screening panel.
Sekbio's sulfonamide residue antibody offering covers the two reagents that matter most for comprehensive sulfonamide-class screening: the Sulfonamide Group Total Antibody, which is broadly cross-reactive with 22 different sulfonamide drugs (overall IC50 2 ppb, ELISA sensitivity 0.3 ppb, colloidal-gold sensitivity range 2–10 ppb across the class) and is calibrated to the sensitivity benchmark set by China's Ministry of Agriculture Announcement No. 235 — the sulfamethazine residue monitoring standard, under which colloidal-gold test-strip format can reach 2 ppb sensitivity specifically for sulfamethazine; and the Trimethoprim (TMP) Antibody (IC50 0.2 ppb, ELISA sensitivity 0.5 ppb, colloidal-gold sensitivity 1–10 ppb). Both reagents are supplied as a matched monoclonal antibody and hapten-BSA conjugate pair, reference priced at $895.52 per mg.
Broad-spectrum group detection plus the sulfonamide synergist trimethoprim, covering the most frequently monitored antibacterial residue class in global food safety programs.
| Target | Class | IC50 | ELISA Sensitivity | LFA Sensitivity | Key Cross-reactivity | Price (mAb or Conjugate, 1mg) |
|---|---|---|---|---|---|---|
| Sulfonamide Group Total | Sulfonamide (broad) | 2 ppb | 0.3 ppb | 2–10 ppb | 22 sulfonamide drugs (incl. sulfamethazine) | $895.52/mg |
| Trimethoprim (TMP) | Sulfonamide Synergist | 0.2 ppb | 0.5 ppb | 1–10 ppb | Co-formulated with sulfonamides | $895.52/mg |
IC50 and sensitivity values from COA release testing in competitive ELISA format. The Sulfonamide Group Total Antibody's colloidal-gold sensitivity is calibrated to the 2 ppb sulfamethazine benchmark set by China's Ministry of Agriculture Announcement No. 235. Prices are reference list prices per 1 mg of monoclonal antibody or hapten-BSA conjugate; OEM bulk-scale pricing for 10 mg to gram quantities is quoted on request. Contact info@sekbio.com for full performance data, sample matrix compatibility, and OEM supply pricing.
Broad-spectrum group screening plus synergist coverage, validated against China's national sulfamethazine residue monitoring standard.
Sekbio's Sulfonamide Group Total Antibody is broadly cross-reactive with 22 different sulfonamide drugs — including sulfamethazine, sulfadiazine, and sulfamethoxazole among the most widely used veterinary members of the class — with overall IC50 of 2 ppb and ELISA sensitivity of 0.3 ppb. This class-wide approach means a single competitive ELISA or LFA test can flag sulfonamide-class contamination regardless of which specific compound is present, which is the practical necessity given how many individual sulfonamide drugs are in veterinary circulation.
China's Ministry of Agriculture Announcement No. 235 establishes the sulfamethazine residue monitoring standard used in Chinese veterinary drug residue testing, under which colloidal-gold test-strip format can reach a sensitivity of 2 ppb specifically for sulfamethazine. Sekbio's Sulfonamide Group Total Antibody colloidal-gold sensitivity range of 2–10 ppb across the class directly encompasses this 2 ppb benchmark, supporting kit developers building test strips intended for China-market regulatory compliance.
Trimethoprim (TMP) is a synergist routinely co-administered with sulfonamides — as in sulfamethoxazole-trimethoprim combination products — to enhance antibacterial activity through a complementary mechanism of action. Sekbio's trimethoprim monoclonal antibody (IC50 0.2 ppb, ELISA sensitivity 0.5 ppb, colloidal-gold sensitivity 1–10 ppb) enables dedicated TMP screening alongside the sulfonamide group antibody, so kit developers can build a combined sulfonamide/TMP panel that mirrors how these drugs are actually used together in the field.
Because sulfonamides are used at high volume across livestock, poultry, and aquaculture, they are among the most frequently detected antibiotic residue classes in food safety monitoring programs worldwide. This makes reliable, low-ppb sulfonamide screening a core requirement — rather than a niche add-on — for any comprehensive veterinary drug residue testing program, and a natural companion to broader antibiotic panels such as Sekbio's Antibiotic Residue Antibodies.
Honey is one of the food matrices most commonly associated with sulfonamide contamination globally, reflecting both intentional veterinary use in beekeeping and cross-contamination risk from surrounding agricultural environments. Sekbio's Sulfonamide Group Total Antibody's ELISA sensitivity of 0.3 ppb supports honey residue ELISA kit development for producers and importers subject to strict sulfonamide-residue import controls.
Sulfonamide use in fish and shrimp farming makes aquaculture a key application matrix for this panel. Sekbio's sulfonamide and trimethoprim antibodies support export compliance testing for aquaculture producers in major seafood-exporting regions, where sulfonamide residue violations are a recurring category of import rejection and border notification in destination markets with strict MRL enforcement.
Sulfonamides are used in dairy cattle for treating bacterial infections, making sulfonamide screening a relevant complement to standard β-lactam and tetracycline milk testing programs. Sekbio's sulfonamide group antibody supports rapid ELISA and LFA screening at the processing plant and farm-gate level, helping dairy operators identify sulfonamide-contaminated milk before it enters the broader supply chain.
MOQ 1 mg per antibody for R&D evaluation. OEM quantities from 10 mg to gram scale are available for food safety kit manufacturing. Sekbio's ISO 13485-certified Shenzhen facility provides quality-assured sulfonamide residue antibody production with lot COA documentation. Contact our team or explore our full antibody catalog for multi-target food safety panel development, including complementary Aminoglycoside Residue Antibodies and Macrolide & Polyether Antibiotic Residue Antibodies.
Broad-spectrum competitive immunoassay antibodies for sulfonamide and trimethoprim residue screening in honey, meat, milk, aquaculture, and feed.
Sulfonamides are one of the most common contaminants detected in honey worldwide, whether from direct veterinary use in beekeeping or environmental cross-contamination. Sekbio's Sulfonamide Group Total Antibody, with ELISA sensitivity of 0.3 ppb and broad 22-drug cross-reactivity, supports comprehensive honey residue screening without requiring a separate test for each individual sulfonamide compound — critical for honey exporters facing strict destination-market import controls.
Sulfonamides are used across cattle, swine, and poultry production for bacterial disease treatment, making broad livestock residue screening a core application for this panel. The Sulfonamide Group Total Antibody's 22-drug cross-reactivity allows a single competitive ELISA or LFA test to screen meat and muscle tissue for the sulfonamide class as a whole, streamlining slaughterhouse and processing-plant testing workflows compared to compound-specific assays.
Sulfonamide use in dairy cattle disease treatment makes milk a relevant sulfonamide residue testing matrix alongside the more commonly screened β-lactam and tetracycline classes. Sekbio's sulfonamide and trimethoprim antibodies enable dairy processors and farm-gate testing programs to add sulfonamide-class screening to existing milk safety testing workflows.
Sulfonamide use in fish and shrimp farming creates residue risk in seafood destined for export markets with strict antibacterial residue import controls. Sekbio's sulfonamide group and trimethoprim antibodies support rapid, low-ppb screening of farmed shrimp, fish, and other aquaculture products, helping producers in major seafood-exporting regions identify non-compliant lots before export shipment.
Verifying that sulfonamide-class compounds have not been incorporated into animal feed outside of authorized veterinary use is an important upstream control point in the residue prevention chain. Screening finished feed and feed premixes with Sekbio's sulfonamide group antibody allows feed manufacturers to catch formulation issues before contaminated feed reaches livestock, poultry, or aquaculture operations.
Technical and commercial questions from food safety IVD developers and kit manufacturers building sulfonamide residue screening assays.
Sulfonamide residue antibodies are monoclonal antibodies raised against sulfonamide drug haptens conjugated to a carrier protein such as BSA. Sulfonamides are small synthetic molecules, so they are detected using competitive inhibition immunoassay formats: free sulfonamide residue in a food sample competes with hapten-protein conjugate for a fixed amount of antibody, producing a signal that decreases as residue concentration increases. This format underlies competitive ELISA kits and colloidal-gold lateral flow (LFA) rapid test strips used across food safety laboratories, veterinary drug residue monitoring programs, and honey, meat, milk, and aquaculture export testing worldwide.
Sulfonamides are not banned — they are a large class of synthetic antibacterial drugs widely and legally used in veterinary medicine across livestock, poultry, and aquaculture, and they are regulated with maximum residue limits (MRLs) in most jurisdictions rather than prohibited outright. Because of their broad, high-volume veterinary use, sulfonamides are among the most frequently detected antibiotic residue classes in food safety monitoring programs globally, which is why a broad-spectrum group screening antibody — rather than single-compound testing — is the standard approach for sulfonamide residue surveillance.
The sulfonamide drug class includes numerous individual compounds — such as sulfamethazine, sulfadiazine, and sulfamethoxazole — that share a common core structure and are often used interchangeably or in rotation in veterinary practice. Testing for each compound individually would require a separate antibody and assay for every sulfonamide drug, which is impractical for routine screening. Sekbio's Sulfonamide Group Total Antibody is broadly cross-reactive with 22 different sulfonamide drugs (IC50 2 ppb, ELISA sensitivity 0.3 ppb, colloidal-gold sensitivity 2–10 ppb across the class), allowing a single competitive ELISA or LFA test to flag any sulfonamide-class residue in one screening step, with compound-specific confirmation reserved for positive samples.
China's Ministry of Agriculture Announcement No. 235 establishes the sulfamethazine residue monitoring standard used in Chinese veterinary drug residue testing programs. Per this standard, colloidal-gold test-strip format can reach a sensitivity of 2 ppb specifically for sulfamethazine. Sekbio's Sulfonamide Group Total Antibody is designed to meet this benchmark, with its colloidal-gold sensitivity range of 2–10 ppb across the sulfonamide class encompassing the 2 ppb sulfamethazine performance level referenced in Announcement No. 235, supporting kit developers building China-market-compliant rapid test strips.
Trimethoprim (TMP) is a synergist commonly co-administered with sulfonamides — as in sulfamethoxazole-trimethoprim combination products — to enhance antibacterial activity by blocking a different step in bacterial folate synthesis than sulfonamides do. Because TMP is so frequently formulated and used together with sulfonamide drugs in veterinary medicine, it is tested alongside the sulfonamide class in residue monitoring panels rather than as an isolated target. Sekbio's trimethoprim monoclonal antibody achieves IC50 of 0.2 ppb, ELISA sensitivity of 0.5 ppb, and colloidal-gold sensitivity of 1–10 ppb.
Sekbio's sulfonamide and trimethoprim antibodies are used across five primary matrices: Honey, where sulfonamides are one of the most common contaminants detected globally; Meat and muscle tissue, for broad livestock residue screening across cattle, swine, and poultry; Milk, given sulfonamide use in dairy cattle disease treatment; Aquaculture and shrimp, reflecting sulfonamide use in fish and shrimp farming; and Animal feed, to verify sulfonamide-class compounds have not been incorporated outside authorized use. Contact info@sekbio.com for matrix-specific extraction protocols and cross-reactivity data across the 22-drug sulfonamide group.
Reference pricing is $895.52 per mg for both the Sulfonamide Group Total Antibody and the Trimethoprim (TMP) Antibody, covering either the monoclonal antibody or its matching hapten-BSA conjugate (1 mg each). MOQ is 1 mg per reagent for R&D evaluation and assay development; OEM bulk-scale pricing for 10 mg to gram quantities is quoted on request. Contact info@sekbio.com for a formal quotation and current lead times.
Yes. Both the Sulfonamide Group Total Antibody and the Trimethoprim Antibody are validated for competitive ELISA (sensitivity 0.3 ppb and 0.5 ppb respectively) and colloidal-gold LFA rapid test strip format (sensitivity 2–10 ppb for the sulfonamide group and 1–10 ppb for trimethoprim). This dual-format validation supports both laboratory-grade quantitative ELISA kit development and field-deployable rapid test strips built on the same antibody-hapten conjugate pair. Contact info@sekbio.com for LFA-specific conjugation protocols and nitrocellulose membrane striping guidance.
For related veterinary drug residue targets, see Sekbio's Antibiotic Residue Antibodies, Aminoglycoside Residue Antibodies, and Macrolide & Polyether Antibiotic Residue Antibodies product pages.
Request sulfonamide and trimethoprim antibody evaluation kits, hapten conjugates, or discuss OEM food safety ELISA and LFA kit development support.