Dexamethasone, Estradiol, Testosterone, Nandrolone, Progesterone & DES Antibodies for Growth-Promoter Residue Screening
Hormone and steroid residue antibodies are monoclonal antibodies raised against natural or synthetic steroid hormone haptens — or, in the case of the non-steroidal tranquilizer chlorpromazine, against the intact drug hapten — conjugated to an immunogenic carrier protein such as BSA. Because these target analytes are small molecules (well under 1,000 Da), they cannot be detected with sandwich immunoassay formats; instead, competitive inhibition ELISA and lateral flow (LFA) formats are used, in which free hormone or drug residue in the sample competes with a hapten-protein conjugate for a limited amount of antibody. High residue concentration produces a weak signal — the inverse relationship characteristic of competitive assays — enabling ppb-level detection suitable for regulatory screening.
Natural and synthetic steroid hormones — estradiol, testosterone, methyltestosterone, and nandrolone (19-nortestosterone) — are banned as growth promoters in food-producing animals across the European Union and China, though the United States permits certain hormonal growth implants under defined regulatory controls. Corticosteroids such as dexamethasone remain in legitimate therapeutic veterinary use for inflammation, allergic reactions, and shock, but are restricted with maximum residue limits because residues carry immunosuppressive and metabolic risks if consumed by humans. Diethylstilbestrol (DES), a synthetic non-steroidal estrogen, has been banned worldwide in food animals since the 1970s–1980s following extensive documentation of carcinogenicity in humans exposed prenatally — making it one of the most consequential cautionary examples in the history of hormone regulation. Chlorpromazine, a phenothiazine-class antipsychotic and sedative, is grouped and screened alongside hormone residues because it is sometimes misused as an illicit livestock tranquilizer to calm animals before transport or slaughter.
Sekbio's hormone and steroid residue antibody panel covers eight targets spanning corticosteroids, natural and synthetic sex steroids, and the phenothiazine tranquilizer class: Dexamethasone (IC50 0.05 ppb, cross-reactive with hydrocortisone, betamethasone, and fludrocortisone; ELISA sensitivity 0.1 ppb; colloidal-gold LFA sensitivity 0.2 ppb); Estradiol (IC50 0.8 ppb, ELISA sensitivity 0.5 ppb); Methyltestosterone (IC50 0.3 ppb, detects commonly used androgens as a class; ELISA sensitivity 0.2 ppb); 19-Nortestosterone/Nandrolone (IC50 0.2 ppb); Testosterone (IC50 0.2 ppb); Progesterone (IC50 1 ppb, ELISA sensitivity 0.1 ppb, colloidal-gold sensitivity 5 ppb); Diethylstilbestrol/DES (IC50 0.34 ppb, ELISA sensitivity 0.05 ppb, colloidal-gold sensitivity 1 ppb); and Chlorpromazine Hydrochloride (IC50 0.1 ppb, cross-reactive with common phenothiazine-class drugs, ELISA sensitivity 0.05 ppb, colloidal-gold sensitivity 0.5 ppb). Each target is supplied as a matched monoclonal antibody and hapten-BSA conjugate pair, reference priced at $895.52 per mg.
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Eight hormone, steroid, and tranquilizer targets covering the major growth-promoter and residue classes monitored in global food safety programs.
| Target | Class | IC50 | ELISA Sensitivity | LFA Sensitivity | Key Cross-reactivity | Price (mAb or Conjugate, 1mg) |
|---|---|---|---|---|---|---|
| Dexamethasone | Corticosteroid | 0.05 ppb | 0.1 ppb | 0.2 ppb | Hydrocortisone, Betamethasone, Fludrocortisone | $895.52/mg |
| Estradiol (E2) | Natural Estrogen | 0.8 ppb | 0.5 ppb | Contact us | Class-specific | $895.52/mg |
| Methyltestosterone | Androgen | 0.3 ppb | 0.2 ppb | Contact us | Detects androgen class | $895.52/mg |
| 19-Nortestosterone (Nandrolone) | Anabolic Steroid | 0.2 ppb | Contact us | Contact us | Class-specific | $895.52/mg |
| Testosterone | Natural Androgen | 0.2 ppb | Contact us | Contact us | Class-specific | $895.52/mg |
| Progesterone | Natural Progestin | 1 ppb | 0.1 ppb | 5 ppb | Class-specific | $895.52/mg |
| Diethylstilbestrol (DES) | Synthetic (Non-Steroidal) Estrogen | 0.34 ppb | 0.05 ppb | 1 ppb | Banned globally since 1970s–1980s | $895.52/mg |
| Chlorpromazine HCl | Phenothiazine Tranquilizer | 0.1 ppb | 0.05 ppb | 0.5 ppb | Common phenothiazine-class drugs | $895.52/mg |
IC50 and sensitivity values from COA release testing in competitive ELISA format. Prices are reference list prices per 1 mg of monoclonal antibody or hapten-BSA conjugate; OEM bulk-scale pricing for 10 mg to gram quantities is quoted on request. Contact info@sekbio.com for full performance data for all targets, sample matrix compatibility, and OEM supply pricing.
Sub-ppb sensitivity across corticosteroids, natural and synthetic sex steroids, and the phenothiazine tranquilizer class for growth-promoter compliance screening.
Sekbio's dexamethasone monoclonal antibody achieves the lowest IC50 in this panel at 0.05 ppb, with ELISA sensitivity of 0.1 ppb and colloidal-gold LFA sensitivity of 0.2 ppb. Deliberate cross-reactivity with hydrocortisone, betamethasone, and fludrocortisone means the antibody detects the broader corticosteroid drug class in a single test — valuable because multiple corticosteroids may be used interchangeably in veterinary therapy, and class-level screening simplifies residue monitoring compared to compound-by-compound testing.
Estradiol is the principal natural estrogen implicated in growth-promoter misuse cases across the EU and China's total ban on hormonal growth promoters. Sekbio's estradiol monoclonal antibody (IC50 0.8 ppb, ELISA sensitivity 0.5 ppb) supports competitive ELISA screening for illicit estradiol use in meat, muscle, and liver tissue, complementing the panel's synthetic-hormone and androgen-class antibodies for comprehensive sex-steroid coverage.
Testosterone and 19-nortestosterone (nandrolone) both achieve IC50 of 0.2 ppb, while methyltestosterone (IC50 0.3 ppb, ELISA sensitivity 0.2 ppb) is designed to detect commonly used androgens as a class. Together, these three antibodies give kit developers androgen-class coverage for the anabolic steroids most frequently associated with illegal growth-promotion in cattle, swine, and poultry — all of which are banned growth-promoting agents under EU and Chinese regulation.
Sekbio's progesterone monoclonal antibody (IC50 1 ppb, ELISA sensitivity 0.1 ppb, colloidal-gold LFA sensitivity 5 ppb) rounds out natural sex-steroid coverage alongside estradiol and testosterone. Progesterone-based growth implants and estrous-synchronization products are used in some livestock production systems, making progestin residue screening a relevant complement to estrogen and androgen monitoring in comprehensive hormone panels.
Diethylstilbestrol has been banned worldwide in food-producing animals since the 1970s–1980s due to well-documented carcinogenicity in humans exposed prenatally, yet continued monitoring remains standard practice because illicit historical use patterns persist in some regions. Sekbio's DES monoclonal antibody delivers IC50 of 0.34 ppb, ELISA sensitivity of 0.05 ppb, and colloidal-gold LFA sensitivity of 1 ppb — sensitive enough for both laboratory confirmation and rapid field screening in meat and seafood/aquaculture matrices.
Chlorpromazine, a phenothiazine-class antipsychotic and sedative, is included in this hormone panel because of its history of misuse as an illicit livestock tranquilizer administered before transport or slaughter. Sekbio's chlorpromazine hydrochloride antibody (IC50 0.1 ppb, ELISA sensitivity 0.05 ppb, colloidal-gold LFA sensitivity 0.5 ppb) is cross-reactive with common phenothiazine-class drugs, supporting broader tranquilizer-class residue screening alongside hormone testing in a single veterinary residue panel.
This panel is built for the sample types where growth-promoter and tranquilizer residues are actually monitored: meat, muscle, and liver tissue at slaughterhouse and processing levels; urine for farm-gate and live-animal screening before an animal enters the food chain; seafood and aquaculture products, reflecting the historical relevance of DES-class compounds in fish and shrimp farming; and animal feed, to verify unauthorized hormonal or tranquilizer compounds have not been incorporated into feed formulations.
MOQ 1 mg per antibody for R&D evaluation. OEM quantities from 10 mg to gram scale are available for food safety kit manufacturing. Sekbio's ISO 13485-certified Shenzhen facility provides quality-assured hormone and steroid residue antibody production with lot COA documentation, serving food safety laboratories and kit manufacturers building growth-promoter compliance programs. Contact our team or explore our full antibody catalog for multi-target food safety panel development support.
Sub-ppb competitive immunoassay antibodies for growth-promoter and tranquilizer residue screening in meat, live animals, seafood, and feed.
Growth-promoter compliance screening in meat, muscle, and liver tissue is a core requirement under the EU's total ban on hormonal growth promoters in food-producing animals (Council Directive 96/22/EC) and equivalent Chinese regulation. Sekbio's estradiol, testosterone, methyltestosterone, and nandrolone antibodies enable competitive ELISA and LFA screening of beef, pork, and poultry tissue for illegal hormone use, while the dexamethasone antibody supports corticosteroid residue monitoring in therapeutically treated animals. Rapid immunoassay screening allows slaughterhouse and processing-plant testing well ahead of the multi-day turnaround typical of LC-MS/MS confirmation.
Several steroid hormones and their metabolites are excreted in urine at concentrations detectable well before slaughter, making urine screening a valuable farm-gate and pre-transport compliance tool. Testing live animals for estradiol, testosterone, nandrolone, and chlorpromazine residues allows producers and inspectors to identify non-compliant animals earlier in the supply chain, reducing the risk of rejected carcasses and regulatory violations discovered only after processing.
Diethylstilbestrol (DES) has a documented history of use in aquaculture and fish farming despite its worldwide ban, reflecting its low cost and effectiveness as an illicit growth promoter in regions with weaker enforcement. Sekbio's DES antibody (ELISA sensitivity 0.05 ppb) supports sensitive screening of farmed shrimp, fish, and other aquaculture products destined for export markets with strict hormone-residue import controls.
Unauthorized incorporation of hormonal growth promoters or tranquilizers into animal feed is a recognized pathway for residue contamination further down the supply chain. Screening finished feed and feed premixes with Sekbio's hormone and chlorpromazine antibodies allows feed manufacturers and regulators to verify formulation compliance before contaminated feed reaches livestock, addressing the root cause of residue violations rather than only the downstream meat or milk product.
Technical and commercial questions from food safety IVD developers and kit manufacturers building hormone and steroid residue screening assays.
Hormone and steroid residue antibodies are monoclonal antibodies raised against natural or synthetic steroid hormone haptens — or, for the non-steroidal tranquilizer chlorpromazine, against the intact drug hapten — conjugated to a carrier protein such as BSA to elicit an immune response. Because these analytes are small molecules, they are detected using competitive inhibition immunoassay formats: free hormone or drug residue in the sample competes with hapten-protein conjugate for a fixed amount of antibody, so a high residue concentration produces a weak signal (positive result) rather than a strong one. This format is used in competitive ELISA kits, colloidal-gold lateral flow (LFA) rapid test strips, and immunoaffinity cleanup columns for screening meat, liver, urine, seafood, and animal feed for growth-promoting hormone residues and tranquilizer misuse ahead of confirmatory LC-MS/MS testing.
Natural and synthetic steroid hormones used as growth promoters — including estradiol, testosterone, methyltestosterone, and nandrolone (19-nortestosterone) — are banned as growth-promoting agents in food-producing animals in the European Union and in China, while the United States permits certain hormonal growth implants under specific regulatory controls. The EU's ban dates to Council Directive 96/22/EC, which prohibits the administration of substances having a hormonal or thyrostatic action to livestock for growth-promotion purposes. Diethylstilbestrol (DES), a synthetic non-steroidal estrogen, is banned worldwide in food animals since the 1970s–1980s due to well-documented carcinogenicity in humans exposed prenatally. Dexamethasone and other corticosteroids remain in legitimate therapeutic veterinary use but are strictly regulated with maximum residue limits because residues can have immunosuppressive and metabolic effects if consumed.
Yes. Sekbio's dexamethasone monoclonal antibody (IC50 0.05 ppb) is intentionally cross-reactive with hydrocortisone, betamethasone, and fludrocortisone — meaning it detects the broader corticosteroid drug class rather than dexamethasone alone. This class-level detection is analytically useful for veterinary residue screening panels, since multiple corticosteroids may be used therapeutically in livestock and a single antibody that flags any member of the class simplifies first-pass screening. ELISA sensitivity is 0.1 ppb and colloidal-gold (LFA) sensitivity is 0.2 ppb, giving kit developers both quantitative and rapid-strip options built on the same antibody.
Diethylstilbestrol (DES) was withdrawn from human medical use and banned in food-producing animals worldwide in the 1970s–1980s after it was linked to carcinogenicity in humans exposed prenatally — one of the most extensively documented cases of a synthetic hormone causing multi-generational health harm. Despite the long-standing ban, DES testing remains part of routine food safety monitoring programs because illegal historical use patterns persist in some regions, particularly in aquaculture and livestock fattening, and because DES is inexpensive and effective as an illicit growth promoter where enforcement is weak. Sekbio's DES monoclonal antibody achieves IC50 of 0.34 ppb, ELISA sensitivity of 0.05 ppb, and colloidal-gold LFA sensitivity of 1 ppb, supporting sensitive screening in meat and seafood/aquaculture matrices.
Chlorpromazine is a first-generation antipsychotic and sedative belonging to the phenothiazine drug class, developed for human and veterinary use to manage anxiety and agitation. It is grouped and screened alongside hormone residues in veterinary residue monitoring panels because it has historically been misused as an illicit livestock tranquilizer — administered before transport or slaughter to reduce stress-related weight loss, bruising, and animal handling difficulty, which constitutes both an animal welfare concern and an unauthorized veterinary drug residue issue. Sekbio's chlorpromazine hydrochloride monoclonal antibody (IC50 0.1 ppb) is cross-reactive with other common phenothiazine-class drugs, with ELISA sensitivity of 0.05 ppb and colloidal-gold LFA sensitivity of 0.5 ppb.
Sekbio's hormone and steroid residue antibodies are used across four primary sample types: Meat, muscle, and liver tissue for growth-promoter compliance screening under the EU's total ban on hormonal growth promoters and equivalent Chinese regulations; Urine and live-animal screening, which allows farm-level or slaughterhouse-gate testing before an animal enters the food chain, since several steroid hormones and their metabolites are excreted in urine at detectable concentrations; Seafood and aquaculture products, reflecting the historical and continuing relevance of DES and other growth promoters in fish and shrimp farming; and Animal feed, to verify that unauthorized hormonal growth promoters or tranquilizers have not been incorporated into feed formulations. Contact info@sekbio.com for matrix-specific extraction and sample preparation protocols.
Reference pricing is $895.52 per mg across all eight targets on this page — dexamethasone, estradiol, methyltestosterone, 19-nortestosterone (nandrolone), testosterone, progesterone, diethylstilbestrol (DES), and chlorpromazine hydrochloride — covering either the monoclonal antibody or its matching hapten-BSA conjugate (1 mg each). MOQ is 1 mg per reagent for R&D evaluation and assay development; OEM bulk-scale pricing for 10 mg to gram quantities is quoted on request. Contact info@sekbio.com for a formal quotation and current lead times.
Yes. All eight hormone and steroid targets in this panel are validated for competitive ELISA, and five — dexamethasone, progesterone, diethylstilbestrol (DES), and chlorpromazine — have documented colloidal-gold LFA sensitivity data (0.2 ppb, 5 ppb, 1 ppb, and 0.5 ppb respectively), enabling both laboratory-grade quantitative ELISA kits and field-deployable rapid test strips from the same antibody-hapten pair. Contact info@sekbio.com for LFA-specific conjugation protocols and colloidal gold particle size recommendations for the remaining targets.
For related veterinary drug residue targets, see Sekbio's Antibiotic Residue Antibodies, Sulfonamide Residue Antibodies, and Beta-Agonist Residue Antibodies product pages.
Request hormone and steroid residue antibody evaluation kits, hapten conjugates, or discuss OEM food safety ELISA and LFA kit development support.