Clenbuterol, Ractopamine, Salbutamol, Phenylethanolamine A & 17-Drug Group Antibodies for Food Safety ELISA & LFA IVD Development
Beta-agonist residue antibodies are monoclonal antibodies raised against beta-agonist drug haptens — small-molecule beta-2 adrenergic agonist compounds conjugated to carrier proteins (BSA or OVA) to generate an immune response suitable for hybridoma production. Beta-agonists such as clenbuterol, salbutamol, ractopamine, and phenylethanolamine A were originally developed as human bronchodilator drugs, but at supra-therapeutic doses in livestock feed they act as "repartitioning agents" — shifting metabolic energy away from fat deposition toward lean muscle growth. Because the resulting drug residues concentrate in liver, lung, retina, and muscle tissue and persist into the retail meat supply, a single contaminated meal can deliver a pharmacologically active dose to a consumer, triggering acute symptoms including tachycardia, tremor, and elevated blood pressure. Competitive ELISA and lateral flow immunoassay (LFA) formats — where free beta-agonist in a sample competes with a beta-agonist-BSA conjugate for antibody binding — allow slaughterhouses, customs inspectors, and feed manufacturers to screen for these compounds at parts-per-billion (ppb) sensitivity without laboratory-grade instrumentation.
Beta-agonists are illegal or tightly restricted feed additives worldwide. Clenbuterol, salbutamol, and phenylethanolamine A are banned outright as livestock growth promoters in China, the EU, and the large majority of food-exporting nations; China's repeated public health incidents tied to contaminated pork gave rise to the widely used term "瘦肉精" ("lean meat powder") for this category of illegal residue. Ractopamine occupies a more contested regulatory position: it is approved for use in swine and cattle feed in the United States and a number of other markets, yet it remains banned in China and the EU and is a standard zero-tolerance screening target at their borders. This jurisdiction-by-jurisdiction variation is exactly why food exporters, meat processors, and import inspection labs need antibody panels that can screen broadly across the entire beta-agonist class rather than a single compound — a shipment that is compliant for one destination market can be an outright violation in another.
Sekbio's beta-agonist residue antibody line covers five reagents built for this exact screening challenge. Phenylethanolamine A (IC50 0.8 ppb, ELISA sensitivity 1 ppb) shows less than 1% cross-reactivity with ractopamine, enabling clean differentiation between the two compounds. Clenbuterol Hydrochloride (IC50 1 ppb, ELISA sensitivity 0.1 ppb, colloidal-gold LFA sensitivity 3 ppb), Salbutamol (IC50 2 ppb, ELISA sensitivity 0.2 ppb, LFA sensitivity 3 ppb), and Ractopamine (IC50 1 ppb, ELISA sensitivity 0.5 ppb, LFA sensitivity 3 ppb) each provide drug-specific quantification for confirmatory testing. For broad-class screening, the Beta-Agonist Group Antibody is cross-reactive with 17 different beta-agonist drugs, with IC50 of 0.3–1 ppb, LFA sensitivity down to 0.015–0.3 ppb, and ELISA sensitivity of 0.02 ppb — a matching Beta-Agonist-OVA antigen is also available. This combination of a broad-spectrum group screen plus compound-specific confirmatory antibodies mirrors how food safety laboratories actually structure their beta-agonist testing programs.
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Every antibody pair is pre-validated on the platform you actually use — LFA, ELISA, or CLIA. Go from catalog to assay in days, not months.
Five reagents covering single-drug quantification and broad-spectrum 17-drug group screening for illegal beta-agonist residues in livestock and meat products.
| Target | IC50 | ELISA Sensitivity | LFA Sensitivity | Key Cross-reactivity | Price (mAb or Conjugate, 1mg) |
|---|---|---|---|---|---|
| Phenylethanolamine A | 0.8 ppb | 1 ppb | Contact us | <1% with ractopamine | $895.52/mg |
| Clenbuterol Hydrochloride | 1 ppb | 0.1 ppb | 3 ppb | Class-specific | $626.87/mg |
| Salbutamol | 2 ppb | 0.2 ppb | 3 ppb | Class-specific | $626.87/mg |
| Ractopamine | 1 ppb | 0.5 ppb | 3 ppb | Class-specific | $626.87/mg |
| Beta-Agonist Group Antibody | 0.3–1 ppb | 0.02 ppb | 0.015–0.3 ppb | 17 beta-agonist drugs | $895.52/mg |
IC50 and sensitivity values from COA release testing in competitive ELISA format. Prices are reference list prices per 1 mg of monoclonal antibody or matching hapten-BSA/OVA conjugate; OEM bulk-scale pricing for 10 mg to gram quantities is quoted on request. Contact info@sekbio.com for full performance data and OEM supply pricing.
Sub-ppb-sensitivity beta-agonist antibodies validated for slaughterhouse, live-animal, feed, and customs screening across the major regulatory jurisdictions.
Sekbio's Beta-Agonist Group Antibody is cross-reactive with 17 different beta-agonist drugs, achieving ELISA sensitivity of 0.02 ppb and colloidal-gold LFA sensitivity as low as 0.015 ppb. This single reagent replaces the need to run 17 separate single-drug assays when the screening goal is to detect any illegal beta-agonist contamination, making it the standard choice for slaughterhouse-line and customs inspection panels. A matching Beta-Agonist-OVA antigen supports both plate-coating ELISA formats and LFA membrane striping.
Sekbio's clenbuterol hydrochloride antibody achieves IC50 of 1 ppb with ELISA sensitivity of 0.1 ppb — among the most sensitive single-drug clenbuterol reagents available — plus colloidal-gold LFA sensitivity of 3 ppb for field-deployable rapid strip testing. Clenbuterol is the beta-agonist most frequently associated with acute human poisoning incidents from contaminated pork and offal, making ultra-sensitive, reliable detection a priority for both domestic food safety monitoring and export compliance.
Sekbio's ractopamine antibody (IC50 1 ppb, ELISA sensitivity 0.5 ppb, LFA sensitivity 3 ppb) supports the zero-tolerance ractopamine screening required by importers such as China and the EU, even where ractopamine is a permitted feed additive at the point of production. Because ractopamine's legal status differs by country, exporters and meat processors serving multiple markets rely on this dedicated antibody to verify shipment-by-shipment compliance rather than assuming a single regulatory standard applies globally.
Sekbio's phenylethanolamine A antibody (IC50 0.8 ppb, ELISA sensitivity 1 ppb) shows cross-reactivity with ractopamine of under 1%, allowing laboratories to confidently distinguish between these two structurally related beta-agonists. This specificity is valuable in confirmatory testing workflows, where a group-antibody screen has flagged beta-agonist contamination and the laboratory needs to identify precisely which compound is present for regulatory reporting.
Sekbio's salbutamol antibody (IC50 2 ppb, ELISA sensitivity 0.2 ppb, LFA sensitivity 3 ppb) provides reliable single-drug quantification for salbutamol, a beta-agonist bronchodilator that — like clenbuterol — has been illegally diverted into livestock feed for growth-promotion purposes. Dedicated single-drug antibodies such as this one complement the broad-spectrum group antibody by giving laboratories a clear quantitative result once group screening indicates a positive sample.
MOQ 1 mg per antibody for R&D evaluation. OEM quantities from 10 mg to gram scale available for food safety kit manufacturing. Sekbio's ISO 13485-certified Shenzhen facility provides quality-assured beta-agonist antibody production with lot COA documentation, serving meat processors, feed manufacturers, and import/export testing laboratories across China, the EU, and Southeast Asia. For broader veterinary drug residue coverage, see our antibiotic residue antibodies and hormone & steroid residue antibodies, or explore the full antibody catalog.
Sub-ppb competitive immunoassay antibodies for slaughterhouse, live-animal, feed, and customs beta-agonist residue screening.
Slaughterhouse screening for illegal beta-agonist residues is a mandatory checkpoint in pork, beef, and mutton production in China and the EU, since beta-agonist residues concentrate in liver and other visceral tissue at higher levels than in muscle. Sekbio's clenbuterol, ractopamine, salbutamol, and Beta-Agonist Group Antibody enable rapid ELISA or LFA screening panels at the slaughter line, reducing the time from sample collection to a hold/release decision from days (for LC-MS/MS confirmation) to under two hours for on-site rapid testing.
Because beta-agonists are excreted largely unmetabolized, urine testing of live animals at the farm or pre-slaughter holding stage is a standard screening point that allows suspect animals to be flagged before they enter the processing chain. Sekbio's Beta-Agonist Group Antibody, with LFA sensitivity down to 0.015–0.3 ppb, supports rapid urine-based colloidal-gold strip testing for on-farm quality control programs.
Illegal beta-agonist adulteration of livestock feed is the root cause of downstream meat contamination, making feed and feed-additive testing a critical control point for feed mills and integrated livestock operations. Sekbio's beta-agonist antibody panel enables feed manufacturers to verify raw material and finished feed batches are free of clenbuterol, ractopamine, salbutamol, phenylethanolamine A, and other group-antibody-reactive compounds before feed reaches farms.
Countries enforcing zero-tolerance beta-agonist policies — including China and the EU — screen incoming meat shipments at customs and border inspection points, since a beta-agonist that is a legal feed additive in the country of origin (such as ractopamine in some markets) can still trigger a shipment rejection at the destination. Sekbio's ractopamine and Beta-Agonist Group Antibody support rapid import-compliance screening for meat processors, importers, and government inspection laboratories. Contact our team for customs-matrix extraction protocols.
Technical and commercial questions from food safety IVD developers and kit manufacturers building beta-agonist residue screening assays.
Beta-agonist residue antibodies are monoclonal antibodies raised against beta-agonist drug haptens conjugated to carrier proteins (BSA/OVA). Beta-agonists — including clenbuterol, salbutamol, ractopamine, and phenylethanolamine A — are synthetic compounds originally developed as bronchodilators for asthma treatment, but when illegally administered to livestock at high doses they act as "repartitioning agents" that shift feed energy from fat deposition to lean muscle growth. Because these residues concentrate in liver, lung, and retina tissue and remain in meat, consuming contaminated pork, beef, or offal can trigger acute cardiovascular symptoms in humans — tachycardia, muscle tremors, elevated blood pressure — even from a single meal. Using a competitive ELISA or lateral flow (LFA) format, free beta-agonist residue in a meat, feed, or urine sample competes with a beta-agonist-BSA conjugate for a limited quantity of antibody: high residue concentration produces a weak or absent signal (positive result). This antibody-based competitive format is what makes ppb-level, on-site slaughterhouse screening for banned beta-agonists practical at scale.
Clenbuterol, salbutamol, and phenylethanolamine A are banned as livestock feed additives in China, the EU, and most food-exporting countries — their use in any food-producing animal is a criminal food safety violation, historically referred to in China as "瘦肉精" (lean meat powder) contamination following repeated public health incidents linked to contaminated pork. Ractopamine has a more fragmented regulatory status: it is approved for use in swine and cattle feed in the United States, Canada, and a number of other countries, but it is banned in China, the EU, and mainland China's major trading partners including Russia and the EU bloc, which enforce zero-tolerance import screening for ractopamine residues in meat. This regulatory patchwork is precisely why food exporters and importers rely on beta-agonist screening panels covering all major compounds rather than a single-drug test — a shipment compliant in one market can be a violation in another.
Sekbio's Beta-Agonist Group Antibody is a broad-spectrum monoclonal antibody engineered for cross-reactivity across 17 different beta-agonist drugs rather than a single compound. It achieves IC50 of 0.3–1 ppb, ELISA sensitivity of 0.02 ppb, and colloidal-gold lateral flow test-strip sensitivity down to 0.015–0.3 ppb — among the most sensitive beta-agonist screening reagents available. A matching Beta-Agonist-OVA antigen is also available for assay development. This group-level detection strategy is the standard approach for slaughterhouse and customs screening, where the goal is to flag any illegal beta-agonist contamination in a single test rather than run 17 separate single-drug assays. Single-drug antibodies (clenbuterol, salbutamol, ractopamine, phenylethanolamine A) remain valuable for confirmatory or drug-specific quantification once a group screen returns positive.
No — cross-reactivity between Sekbio's phenylethanolamine A antibody and ractopamine is under 1%, meaning the two compounds can be reliably distinguished from one another. This specificity is useful when a laboratory needs to confirm which individual beta-agonist is present after a group-antibody screen returns positive, since phenylethanolamine A and ractopamine belong to the same structural class but have different regulatory status in some jurisdictions. The phenylethanolamine A antibody has IC50 0.8 ppb and ELISA sensitivity of 1 ppb.
Sekbio's beta-agonist antibodies are validated for competitive ELISA and LFA testing across the matrices most relevant to livestock beta-agonist screening: Muscle and liver tissue from pork, beef, and mutton at slaughterhouses, since beta-agonist residues concentrate in liver and other visceral organs at higher levels than in muscle; Urine from live animals, used for pre-slaughter and on-farm screening because beta-agonists are excreted largely unmetabolized; Feed and feed additive samples, to verify that illegal beta-agonist adulteration has not occurred during feed manufacturing; and Imported meat at customs inspection points, where importing countries with zero-tolerance policies (including China and the EU) screen incoming shipments for clenbuterol, salbutamol, and ractopamine. Contact info@sekbio.com for matrix-specific extraction protocols.
Reference pricing is $895.52 per mg for Phenylethanolamine A and the broad-spectrum Beta-Agonist Group Antibody (and its matching Beta-Agonist-OVA antigen), and $626.87 per mg for Clenbuterol Hydrochloride, Salbutamol, and Ractopamine — each price covering either the monoclonal antibody or its matching hapten-BSA/OVA conjugate (1 mg each). MOQ is 1 mg per reagent for R&D evaluation; OEM bulk-scale pricing for 10 mg to gram quantities is quoted on request. Contact info@sekbio.com for a formal quotation and current lead times.
Yes. All five beta-agonist antibodies on this page are validated for both competitive ELISA and colloidal-gold lateral flow (LFA) formats. Colloidal-gold test-strip sensitivity is 3 ppb for clenbuterol, salbutamol, and ractopamine, and 0.015–0.3 ppb for the broad-spectrum Beta-Agonist Group Antibody — sensitive enough for rapid on-site screening at slaughterhouses and customs checkpoints without laboratory instrumentation. ELISA format offers higher sensitivity (down to 0.02 ppb for the group antibody) and is preferred for confirmatory or quantitative testing. Contact info@sekbio.com for LFA conjugation protocols and nitrocellulose membrane striping parameters.
Request beta-agonist residue antibody evaluation kits, hapten conjugates, or discuss OEM food safety ELISA and LFA kit development support.