Metronidazole, Dimetridazole, Ronidazole & Albendazole Monoclonal Antibodies for Veterinary Drug Residue ELISA & LFA IVD Development
A nitroimidazole antibody is a monoclonal antibody raised against nitroimidazole drug haptens — small-molecule antiprotozoal/antibacterial compounds conjugated to a carrier protein (typically BSA) — used to build competitive inhibition immunoassays for veterinary drug residue detection. Because nitroimidazole molecules are small, free drug residue in a food or feed sample competes with a nitroimidazole-BSA conjugate for antibody binding sites in ELISA or lateral flow (LFA) format; high residue concentration suppresses signal, producing the positive-result logic that regulatory screening programs rely on. A related but chemically distinct class, benzimidazole anthelmintics such as albendazole, uses the same competitive detection principle but serves a different regulatory purpose: rather than being banned, albendazole is a permitted dewormer subject to Maximum Residue Limit (MRL) compliance testing.
Nitroimidazoles — metronidazole, dimetridazole, ronidazole, and their hydroxy metabolites — are antiprotozoal and antibacterial drugs that were historically used in poultry and pig farming to treat protozoal infections such as histomoniasis and swine dysentery, and in beekeeping to treat honeybee brood diseases. Toxicological research linking nitroimidazoles to genotoxic and carcinogenic effects led to their prohibition in food-producing animals across the EU, United States, and China. Because prohibition does not eliminate demand for cheap, effective antiprotozoal treatment, illegal nitroimidazole use continues to be detected in poultry and honey — making broad, sensitive nitroimidazole residue screening a persistent requirement in food safety testing programs rather than a closed regulatory chapter.
Sekbio's Nitroimidazole Group Antibody is a broadly cross-reactive monoclonal antibody with documented performance across six related compounds: isopropronidazole (IC50 0.5 ppb), dimetridazole (IC50 3 ppb), ronidazole (IC50 5 ppb), hydroxy-isopropronidazole — a metabolite (IC50 13 ppb), hydroxy-dimetridazole — a metabolite (IC50 20 ppb), and metronidazole (IC50 0.1 ppb, the most sensitive target in the panel). This broad cross-reactivity profile allows a single antibody to anchor a multi-compound nitroimidazole screening panel rather than requiring separate antibodies for each parent drug and its hydroxy metabolite — an important practical advantage given that nitroimidazole parent compounds convert to hydroxy metabolites in vivo, and testing programs that ignore metabolites risk under-reporting true residue exposure.
Alongside the nitroimidazole panel, Sekbio supplies a dedicated Albendazole Monoclonal Antibody (IC50 1 ppb) for benzimidazole anthelmintic residue testing. Albendazole is widely used in cattle, sheep, and swine to control parasitic worm infections, and while it carries established MRLs in many jurisdictions rather than an outright ban, exporters and processors must still demonstrate compliant residue levels in meat, liver, and feed. The albendazole antibody is cross-reactive with albendazole-2-aminosulfone, albendazole sulfoxide, and albendazole sulfone — its three principal metabolites — allowing a single assay to capture both the parent drug and its major metabolic products, which is critical given how rapidly albendazole is metabolized in livestock tissue.
Two reagent families covering banned nitroimidazole residues and permitted-but-monitored albendazole anthelmintic residues.
| Target Compound | Class | IC50 | Antibody | Regulatory Status | Price (1 mg) |
|---|---|---|---|---|---|
| Metronidazole (MNZ) | Nitroimidazole | 0.1 ppb | Nitroimidazole Group Ab | Banned in food animals | $895.52/mg |
| Isopropronidazole (IPZ) | Nitroimidazole | 0.5 ppb | Nitroimidazole Group Ab | Banned in food animals | $895.52/mg |
| Dimetridazole (DMZ) | Nitroimidazole | 3 ppb | Nitroimidazole Group Ab | Banned in food animals | $895.52/mg |
| Ronidazole (RNZ) | Nitroimidazole | 5 ppb | Nitroimidazole Group Ab | Banned in food animals | $895.52/mg |
| Hydroxy-Isopropronidazole | Nitroimidazole Metabolite | 13 ppb | Nitroimidazole Group Ab | Banned in food animals | $895.52/mg |
| Hydroxy-Dimetridazole | Nitroimidazole Metabolite | 20 ppb | Nitroimidazole Group Ab | Banned in food animals | $895.52/mg |
| Albendazole (ABZ) | Benzimidazole Anthelmintic | 1 ppb | Albendazole mAb | MRL-regulated (permitted) | $895.52/mg |
| Albendazole Sulfoxide / Sulfone / 2-Aminosulfone | Benzimidazole Metabolites | Cross-reactive at ABZ IC50 | Albendazole mAb | MRL-regulated (permitted) | $895.52/mg |
IC50 values from COA release testing in competitive ELISA format. Prices are reference list prices per 1 mg of monoclonal antibody or matching hapten-BSA conjugate; OEM bulk-scale pricing for 10 mg to gram quantities is quoted on request. Contact info@sekbio.com for full performance data and matrix-specific extraction protocols.
Broad cross-reactivity nitroimidazole antibody plus a dedicated, metabolite-inclusive albendazole antibody for comprehensive banned-drug and MRL-compliance testing.
Sekbio's nitroimidazole group antibody achieves its lowest IC50 — 0.1 ppb — against metronidazole, the most commonly monitored nitroimidazole compound in food safety testing. This ultra-sensitive detection supports confident screening in poultry, honey, and other matrices where illicit metronidazole use has been documented despite its prohibited status.
Rather than requiring six separate antibodies, Sekbio's nitroimidazole group antibody delivers documented IC50 performance across isopropronidazole, dimetridazole, ronidazole, metronidazole, and both major hydroxy metabolites. This consolidates a comprehensive nitroimidazole screening panel onto a single reagent for ELISA or LFA kit development.
Because nitroimidazole parent drugs are metabolized in vivo to hydroxy derivatives, an antibody that only detects the parent compound risks missing residues that have already converted. Sekbio's antibody's documented reactivity with hydroxy-isopropronidazole (13 ppb) and hydroxy-dimetridazole (20 ppb) ensures testing programs capture both parent and metabolite forms.
Sekbio's albendazole monoclonal antibody achieves an IC50 of 1 ppb, providing sensitive detection for this widely used benzimidazole anthelmintic in cattle, sheep, and swine. The antibody supports both quantitative ELISA and rapid LFA screening for MRL-compliance verification in meat and liver samples.
The albendazole antibody's cross-reactivity with albendazole-2-aminosulfone, albendazole sulfoxide, and albendazole sulfone — the drug's three major metabolites — means the assay captures the parent compound and its main metabolic products in a single test, reflecting how rapidly albendazole is metabolized in livestock and providing a more complete picture of total albendazole-related residue.
The combined nitroimidazole/albendazole antibody portfolio spans the two most relevant veterinary drug residue contexts for mixed livestock and apiary operations: banned nitroimidazole compliance in poultry meat, eggs, and honey, and MRL-compliance albendazole testing in cattle, sheep, and swine — covering both prohibited and permitted-but-regulated veterinary drug categories on a shared testing platform.
Both antibodies are supplied together with matching hapten-BSA conjugates suitable for competitive ELISA microplate assays and colloidal-gold lateral flow rapid test strips, supporting kit developers building either laboratory-based quantitative assays or field-deployable qualitative screening tools.
MOQ is 1 mg per antibody for R&D evaluation, with OEM quantities from 10 mg to gram scale available for kit manufacturing. Sekbio's ISO 13485-certified Shenzhen facility supports quality-assured production with lot COA documentation. Explore the full Sekbio antibody catalog or contact our team for veterinary drug residue panel development support.
Broad cross-reactivity antibodies for banned nitroimidazole compliance and MRL-regulated albendazole residue screening across poultry, honey, and livestock matrices.
Nitroimidazoles were historically used in poultry farming to treat protozoal infections such as histomoniasis (blackhead disease) in turkeys and chickens. Because these drugs are banned in food-producing animals across the EU, US, and China, poultry processors and export testing laboratories rely on sensitive nitroimidazole screening — including hydroxy metabolite detection — to demonstrate compliance in muscle, liver, and egg samples before product reaches export or domestic retail channels.
Ronidazole and metronidazole have historically been used illicitly in beekeeping to treat honeybee brood diseases such as European and American foulbrood, despite being banned substances in food production. Honey testing laboratories use nitroimidazole group antibodies to screen for these compounds and their hydroxy metabolites, supporting both domestic quality control and export compliance documentation for honey producers and packers.
Albendazole is a widely used anthelmintic in swine and cattle production for parasitic worm control. Because it carries established, non-zero MRLs rather than an outright ban, processors and testing laboratories use the albendazole antibody — cross-reactive with sulfoxide, sulfone, and 2-aminosulfone metabolites — to verify that appropriate withdrawal periods have been observed and that muscle and liver tissue residues fall within regulatory limits.
Both the nitroimidazole group antibody and the albendazole antibody support animal feed screening programs, which verify that feed formulations do not contain unauthorized nitroimidazole compounds and that albendazole-medicated feed is dosed correctly. Feed-level screening is a preventive control point that reduces the likelihood of downstream residue violations in finished meat, egg, and dairy products.
Technical and commercial questions from food safety IVD developers building veterinary drug residue screening assays.
Nitroimidazoles are a class of antiprotozoal and antibacterial drugs — including metronidazole, dimetridazole, and ronidazole — historically used in poultry and pig farming to treat protozoal and bacterial infections, and in beekeeping to treat honeybee brood diseases. They are banned for use in food-producing animals in the EU, United States, and China due to genotoxic and carcinogenic concerns identified in toxicological research. Because nitroimidazoles are also converted in vivo to hydroxy metabolites that retain toxicological relevance, regulatory testing typically screens for both parent compounds and their hydroxy metabolites.
Sekbio's nitroimidazole group antibody is a broadly cross-reactive monoclonal antibody with documented IC50 values across six nitroimidazole-related compounds: isopropronidazole (0.5 ppb), dimetridazole (3 ppb), ronidazole (5 ppb), hydroxy-isopropronidazole metabolite (13 ppb), hydroxy-dimetridazole metabolite (20 ppb), and metronidazole (0.1 ppb). This broad cross-reactivity allows a single antibody to support a multi-compound nitroimidazole screening panel rather than requiring a separate antibody for each drug and metabolite.
Albendazole is a benzimidazole anthelmintic (dewormer) widely used in livestock to control parasitic worm infections. Unlike nitroimidazoles, albendazole is permitted for veterinary use with established Maximum Residue Limits (MRLs) in many jurisdictions rather than being banned outright. However, because albendazole and its metabolites can persist in tissue and liver, and MRL compliance must still be demonstrated, it and its metabolites are routinely screened as part of standard livestock residue monitoring and export compliance testing programs.
Yes. Sekbio's albendazole monoclonal antibody (IC50 1 ppb) is cross-reactive with albendazole-2-aminosulfone, albendazole sulfoxide, and albendazole sulfone — the three major metabolites formed as albendazole is metabolized in livestock. This means the antibody captures the parent drug and its main metabolites in a single competitive assay, which is analytically important because albendazole is rapidly metabolized in vivo and residual tissue concentrations of the parent compound alone can understate total albendazole-related exposure.
The nitroimidazole group antibody is most relevant to poultry meat and eggs (nitroimidazole ban compliance) and honey (illicit ronidazole and metronidazole use in beekeeping to treat brood diseases). The albendazole antibody is most relevant to swine and cattle meat and liver tissue, where albendazole is used therapeutically and MRL compliance must be demonstrated, as well as animal feed screening for verification of correct dosing and withdrawal period compliance.
Both the nitroimidazole group monoclonal antibody and the albendazole monoclonal antibody are priced at a reference rate of $895.52 per mg, covering either the antibody itself or its matching hapten-BSA conjugate (1 mg each). MOQ is 1 mg per reagent for R&D evaluation, with OEM bulk quantities from 10 mg to gram scale available for ELISA and LFA test kit manufacturing. Contact info@sekbio.com for a formal quotation.
Yes. Both the nitroimidazole group antibody and the albendazole antibody are supplied together with matching hapten-BSA conjugates suitable for competitive ELISA microplate development and colloidal-gold lateral flow (LFA) rapid test strip development. Contact info@sekbio.com for antibody conjugation protocols and nitrocellulose membrane striping parameter guidance specific to nitroimidazole and benzimidazole residue detection.
Nitroimidazole and albendazole testing is frequently paired with other banned veterinary drug targets in shared ELISA/LFA platforms.
Chloramphenicol, streptomycin, tetracycline, and fluoroquinolone residues are routinely co-tested with nitroimidazole compounds in poultry and honey screening panels. See the Antibiotic Residue Antibody hub page for the full veterinary drug residue antibody catalog.
Nitrofuran metabolites (AOZ, SEM, AMOZ, AHD) are another banned veterinary drug class frequently screened alongside nitroimidazoles in poultry and honey. Review the Nitrofuran Metabolite Antibody data sheet for full performance specifications.
Browse the complete Sekbio antibody product catalog or contact our team to discuss multi-target veterinary drug residue panel development.
Request nitroimidazole or albendazole antibody evaluation kits, hapten conjugates, or discuss OEM ELISA and LFA kit development support.