Aflatoxin B1, Aflatoxin M1, Ochratoxin A, DON, Zearalenone & Fumonisin Antibodies for Food Safety ELISA & LFA IVD Development
Mycotoxin antibodies are monoclonal antibodies raised against mycotoxin haptens conjugated to carrier proteins (BSA or OVA), used to develop competitive ELISA and lateral flow (LFA) rapid tests for fungal toxin contamination in food and feed. Mycotoxins are toxic secondary metabolites produced by fungi (mold) that contaminate grains, nuts, dairy, and other agricultural products, especially under warm and humid storage conditions. Because mycotoxin molecules are small, they are detected using competitive inhibition immunoassay formats: free mycotoxin in the sample competes with a mycotoxin-BSA conjugate for a limited amount of antibody, so higher mycotoxin concentration produces weaker signal. This format enables the sub-ppb to low-ppm sensitivity required for regulatory-relevant screening across grain import/export, dairy processing, and animal feed manufacturing.
Several mycotoxins — most notably aflatoxin B1 — are among the most potent naturally occurring carcinogens known, making mycotoxin screening one of the highest-priority categories in global food safety testing. Aflatoxins are produced primarily by Aspergillus species and contaminate corn, peanuts, tree nuts, and other crops both in the field and during storage. Aflatoxin M1 is the hydroxylated metabolite of aflatoxin B1 formed in the liver of dairy animals that consumed AFB1-contaminated feed, making it the standard aflatoxin marker for milk safety testing. Ochratoxin A, produced by Aspergillus and Penicillium species, contaminates grains, coffee, and dried fruit. Deoxynivalenol (DON, commonly called vomitoxin) and zearalenone are produced by Fusarium species and are major contaminants of wheat, barley, and corn. Fumonisins, also Fusarium-derived, primarily contaminate corn. T-2 toxin, another Fusarium mycotoxin, is one of the most acutely toxic trichothecenes. Because mycotoxin contamination in feed passes upstream into dairy products (as AFM1) and can affect multiple crop types simultaneously, comprehensive multi-mycotoxin antibody panels are standard practice across grain, dairy, nut, and feed testing programs.
Sekbio's mycotoxin antibody panel covers eight targets with documented performance data. Aflatoxin B1 (AFB1) — the flagship, highest-search-volume target — has IC50 0.05 ppb, cross-reactivity of 100% for B1, under 20% for B2, and under 5% for G1/G2, ELISA sensitivity of 0.001 ppb, and colloidal-gold sensitivity of 0.3–1 ppb. Aflatoxin M1 (AFM1), the milk-specific metabolite, has IC50 0.02 ppb, ELISA sensitivity 0.002 ppb, and colloidal-gold sensitivity 0.05–0.3 ppb. Total Aflatoxin Antibody detects the full aflatoxin family (B1, B2, G1, G2) with over 80% overall cross-reactivity, ELISA sensitivity 0.001 ppb, and colloidal-gold sensitivity 1 ppb. Ochratoxin A (OTA) has IC50 1 ppb, ELISA sensitivity 0.2 ppb, colloidal-gold sensitivity 1 ppb. DON has IC50 1 ppb, ELISA sensitivity 0.02 ppm, colloidal-gold sensitivity 10 ppb, and cross-reacts with 3-acetyl-DON, 15-acetyl-DON, and masked/conjugated DON forms. Zearalenone (ZEN) has IC50 0.02 ppb, ELISA sensitivity 0.002 ppb, colloidal-gold sensitivity 2 ppb, and cross-reacts with zearalenol (80%). Fumonisin has IC50 2 ppb, ELISA sensitivity 10 ppb, colloidal-gold sensitivity 2 ppb. T-2 Toxin has IC50 0.5 ppb, ELISA sensitivity 2 ppb, colloidal-gold sensitivity 5 ppb. Reference price is $895.52/mg for each target, for either the monoclonal antibody or its matching hapten-BSA conjugate.
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Eight mycotoxin targets covering the aflatoxin family, Fusarium-derived toxins, and Ochratoxin A, each supplied as a monoclonal antibody and matching hapten-BSA conjugate.
| Target Mycotoxin | IC50 | ELISA Sensitivity | Colloidal-Gold Sensitivity | Key Cross-reactivity | Price (mAb or Conjugate, 1mg) |
|---|---|---|---|---|---|
| Aflatoxin B1 (AFB1) | 0.05 ppb | 0.001 ppb | 0.3–1 ppb | B1 100%, B2 <20%, G1/G2 <5% | $895.52/mg |
| Aflatoxin M1 (AFM1) | 0.02 ppb | 0.002 ppb | 0.05–0.3 ppb | M1 100%, M2 <10%, B1/B2 <5% | $895.52/mg |
| Total Aflatoxin | B1 0.03 / B2 0.02 / G1 0.05 / G2 0.05 ppb | 0.001 ppb | 1 ppb | >80% overall (B1/B2/G1/G2) | $895.52/mg |
| Ochratoxin A (OTA) | 1 ppb | 0.2 ppb | 1 ppb | Class-specific | $895.52/mg |
| Deoxynivalenol (DON) | 1 ppb | 0.02 ppm (20 ppb) | 10 ppb | 3-Ac-DON >70%, 15-Ac-DON >60%, Masked-DON >40% | $895.52/mg |
| Zearalenone (ZEN) | 0.02 ppb | 0.002 ppb | 2 ppb | Zearalenol (80%) | $895.52/mg |
| Fumonisin | 2 ppb | 10 ppb | 2 ppb | Class-specific | $895.52/mg |
| T-2 Toxin | 0.5 ppb | 2 ppb | 5 ppb | Class-specific | $895.52/mg |
IC50, ELISA, and colloidal-gold sensitivity values from COA release testing in competitive ELISA and LFA format. Prices are reference list prices per 1 mg of monoclonal antibody or hapten-BSA conjugate; OEM bulk-scale pricing for 10 mg to gram quantities is quoted on request. Contact info@sekbio.com for full performance data for all targets and matrix-specific extraction protocols.
Ultra-sensitive mycotoxin antibodies validated across grain, dairy, nut, and animal feed matrices for the highest-priority contaminant category in food safety testing.
Sekbio's aflatoxin B1 (AFB1) monoclonal antibody achieves IC50 of 0.05 ppb with ELISA detection sensitivity of 0.001 ppb and colloidal-gold LFA sensitivity of 0.3–1 ppb. AFB1 is classified among the most potent naturally occurring carcinogens known, making it the single most heavily regulated and highest-search-volume mycotoxin in global food safety testing. The antibody's high B1-specificity — 100% for B1 versus under 20% for B2 and under 5% for G1/G2 — ensures accurate B1-attributed results without dilution from other aflatoxin family members.
Sekbio's AFM1 antibody (IC50 0.02 ppb, ELISA sensitivity 0.002 ppb) targets the hydroxylated liver metabolite of AFB1 that is excreted into milk when dairy animals consume AFB1-contaminated feed. AFM1 testing is one of the most routine dairy QC tests globally precisely because it reflects upstream feed contamination. With colloidal-gold sensitivity of 0.05–0.3 ppb and high M1-specificity (100% M1 vs. under 10% M2 and under 5% B1/B2), the antibody supports both laboratory ELISA and rapid on-site milk screening.
For applications requiring comprehensive aflatoxin-family screening rather than B1-specific quantification, Sekbio's Total Aflatoxin Antibody is cross-reactive with B1 (IC50 0.03 ppb), B2 (0.02 ppb), G1 (0.05 ppb), and G2 (0.05 ppb), with an overall cross-reactivity rate above 80% across the group. ELISA sensitivity of 0.001 ppb and colloidal-gold sensitivity of 1 ppb make it well suited to grain import screening programs where the goal is to detect any aflatoxin contamination rather than attribute it to a specific family member.
Sekbio's Deoxynivalenol (DON, "vomitoxin") antibody (IC50 1 ppb, ELISA sensitivity 0.02 ppm) cross-reacts with 3-acetyl-DON (>70%), 15-acetyl-DON (>60%), and masked/conjugated DON forms (>40%), in addition to 100% reactivity with parent DON. Because these modified DON forms commonly co-occur with parent DON in Fusarium-contaminated wheat, barley, and corn, and can convert back to DON during digestion, this broad reactivity profile provides a more complete picture of total DON-related exposure than parent-DON-only detection.
Sekbio's zearalenone antibody (IC50 0.02 ppb, ELISA sensitivity 0.002 ppb) cross-reacts with its reduced metabolite zearalenol (80%), while the fumonisin antibody (IC50 2 ppb, ELISA sensitivity 10 ppb) provides class-specific detection. Both are Fusarium-derived mycotoxins that primarily contaminate corn and other cereal grains, and are typically screened alongside DON and aflatoxins in comprehensive grain safety and animal feed testing panels.
Sekbio's T-2 Toxin antibody (IC50 0.5 ppb, ELISA sensitivity 2 ppb, colloidal-gold test-card sensitivity 5 ppb) targets one of the most acutely toxic trichothecene mycotoxins, produced by Fusarium species in stored grain under cool, damp conditions. T-2 toxin screening is a standard component of comprehensive grain and animal feed mycotoxin monitoring programs alongside DON, zearalenone, and the aflatoxins.
Sekbio's Ochratoxin A (OTA) antibody (IC50 1 ppb, ELISA sensitivity 0.2 ppb, colloidal-gold sensitivity 1 ppb) supports OTA screening in grains, coffee, and dried fruit — matrices where Aspergillus and Penicillium mold growth during storage is a well-documented contamination pathway. OTA is typically included alongside aflatoxin testing in comprehensive grain and dried-food safety programs.
MOQ 1 mg per antibody for R&D evaluation. OEM quantities from 10 mg to gram scale available for food safety kit manufacturing. Sekbio's ISO 13485-certified Shenzhen facility provides quality-assured mycotoxin antibody production with lot COA documentation, serving grain importers/exporters, dairy processors, nut and dried fruit handlers, and animal feed manufacturers across EU, Chinese, and Southeast Asian markets. Explore Sekbio's related pesticide residue antibodies and antibiotic residue antibodies for broader food and feed safety panel development, or contact our team for support.
Ultra-sensitive competitive immunoassay antibodies for grain, dairy, nut, and animal feed mycotoxin screening across the aflatoxin, Fusarium, and Ochratoxin categories.
Corn, wheat, and rice are tested for mycotoxin contamination at harvest, during storage, and at import/export checkpoints, given the well-documented risk of fungal growth under warm, humid storage conditions. Sekbio's Total Aflatoxin, DON, Zearalenone, and Fumonisin antibodies together provide comprehensive coverage of the mycotoxins most commonly associated with cereal crop contamination, supporting grain trading companies, mills, and import-control testing laboratories.
Aflatoxin M1 testing is one of the most routine and highest-volume mycotoxin tests in the dairy industry, since AFM1 in milk directly reflects AFB1 contamination in the animal feed consumed by dairy cattle. Sekbio's AFM1 antibody (IC50 0.02 ppb, ELISA sensitivity 0.002 ppb) supports both laboratory ELISA-based confirmatory testing and rapid colloidal-gold screening for dairy processors and milk collection stations.
Peanuts, tree nuts, and dried fruit are classic high-risk matrices for aflatoxin B1 contamination, given the crops' susceptibility to Aspergillus mold growth during growing, harvesting, and storage. Sekbio's AFB1 antibody, with its ultra-low IC50 of 0.05 ppb and ELISA sensitivity of 0.001 ppb, is the primary screening reagent for nut and dried fruit processors and export testing laboratories monitoring this highest-priority mycotoxin.
Animal feed manufacturers screen raw materials and finished feed for multiple mycotoxins before the feed is used, since contaminated feed is the direct upstream source of AFM1 passing into dairy milk, as well as a direct toxicity risk to livestock. Sekbio's Total Aflatoxin, DON, Zearalenone, Fumonisin, Ochratoxin A, and T-2 Toxin antibodies together support comprehensive feed safety testing panels for feed mills and livestock producers.
Technical and commercial questions from food safety IVD developers and kit manufacturers building mycotoxin screening assays.
Mycotoxins are toxic secondary metabolites produced by fungi (mold) that contaminate grains, nuts, dairy, and other agricultural products, particularly under warm and humid storage conditions. Several mycotoxins — most notably aflatoxin B1 — are among the most potent naturally occurring carcinogens known, which is why mycotoxin screening ranks among the highest-priority categories in global food safety testing, spanning grain import/export inspection, dairy processing quality control, and animal feed manufacturing. Because mycotoxin molecules are small, antibody-based competitive ELISA and lateral flow (LFA) immunoassays are the primary rapid screening tool: free mycotoxin in the sample competes with a mycotoxin-BSA conjugate for antibody binding, enabling ppb-to-ppt-level sensitivity without laboratory instrumentation.
Sekbio's aflatoxin B1 antibody has IC50 0.05 ppb, ELISA sensitivity of 0.001 ppb, and colloidal-gold LFA test-strip sensitivity of 0.3–1 ppb. Within the aflatoxin family, the antibody is highly B1-specific: cross-reactivity is 100% for B1 itself, under 20% for aflatoxin B2, and under 5% for aflatoxin G1 and G2. This B1-selective profile matters because aflatoxin B1 is the most toxic and most heavily regulated member of the aflatoxin family, and it is the single highest-search-volume mycotoxin term in food safety testing, given its classification as one of the most potent naturally occurring carcinogens known.
Aflatoxin M1 (AFM1) is the hydroxylated metabolite of aflatoxin B1, formed in the liver of dairy animals that have consumed AFB1-contaminated feed and subsequently excreted into milk. Because AFM1 is structurally distinct from AFB1, a dedicated antibody is required for accurate milk testing. Sekbio's AFM1 antibody has IC50 0.02 ppb — more sensitive than the AFB1 antibody's 0.05 ppb — with ELISA sensitivity of 0.002 ppb and colloidal-gold sensitivity of 0.05–0.3 ppb. Cross-reactivity within the aflatoxin family is 100% for M1, under 10% for M2, and under 5% for B1/B2, making it a milk-specific, AFM1-selective reagent. AFM1 testing is a major routine dairy QC test precisely because it reflects upstream AFB1 contamination in animal feed.
Sekbio's individual AFB1 and AFM1 antibodies are optimized for high specificity to their respective target (B1-specific or M1-specific, respectively), which is useful when a testing program needs to attribute contamination to a particular aflatoxin. The Total Aflatoxin Antibody is instead designed for broad-group detection: it is cross-reactive with aflatoxin B1 (IC50 0.03 ppb), B2 (0.02 ppb), G1 (0.05 ppb), and G2 (0.05 ppb), with an overall cross-reactivity rate above 80% across the group. ELISA sensitivity is 0.001 ppb and colloidal-gold sensitivity is 1 ppb. The Total Aflatoxin Antibody is the preferred choice when the testing goal is comprehensive aflatoxin-family screening — for example, grain import screening — rather than B1-specific or M1-specific quantification.
Yes. Sekbio's Deoxynivalenol (DON, commonly called vomitoxin) antibody has IC50 1 ppb and shows cross-reactivity of over 70% with 3-acetyl-DON, over 60% with 15-acetyl-DON, and over 40% with masked/conjugated DON forms, in addition to 100% reactivity with parent DON. ELISA sensitivity is 0.02 ppm (20 ppb) and colloidal-gold sensitivity is 10 ppb. This broad reactivity across DON's major acetylated and masked derivatives is analytically important because these modified forms can be present alongside parent DON in contaminated grain and can convert back to DON during digestion, so detecting them together provides a more complete picture of total DON-related exposure risk.
For grain and cereal storage/import QC (corn, wheat, rice), the Total Aflatoxin Antibody, DON, Zearalenone, and Fumonisin antibodies provide the broadest relevant coverage, since these are the mycotoxins most commonly associated with field and storage mold contamination in cereal crops. For dairy and milk, the AFM1 antibody is the standard test, since it reflects AFB1 passed through from contaminated animal feed. For nuts and dried fruit — a classic high-risk matrix, particularly peanuts and tree nuts — the AFB1 antibody is the primary target given its potency and regulatory profile. For animal feed manufacturing, comprehensive multi-mycotoxin screening (Total Aflatoxin, DON, Zearalenone, Fumonisin, Ochratoxin A, T-2 Toxin) is recommended before feed is used, since contaminated feed is the upstream source of AFM1 in milk.
Zearalenone (ZEN): IC50 0.02 ppb, ELISA sensitivity 0.002 ppb, colloidal-gold sensitivity 2 ppb, cross-reactive with its reduced metabolite zearalenol (80%). Ochratoxin A (OTA): IC50 1 ppb, ELISA sensitivity 0.2 ppb, colloidal-gold sensitivity 1 ppb. Fumonisin: IC50 2 ppb, ELISA sensitivity 10 ppb, colloidal-gold sensitivity 2 ppb. T-2 Toxin: IC50 0.5 ppb, ELISA sensitivity 2 ppb, colloidal-gold test-card sensitivity 5 ppb. All four antibodies are supplied as monoclonal antibody and matching hapten-BSA conjugate pairs at $895.52/mg, validated for competitive ELISA and LFA development.
MOQ is 1 mg per antibody for R&D evaluation and assay development. Reference price is $895.52 per mg across all eight mycotoxin targets on this page — T-2 Toxin, Aflatoxin B1, Aflatoxin M1, Total Aflatoxin, Ochratoxin A, DON, Zearalenone, and Fumonisin — covering either the monoclonal antibody or its matching hapten-BSA conjugate (1 mg each). OEM quantities from 10 mg to gram scale are available for kit manufacturing, with bulk pricing quoted on request. Contact info@sekbio.com for a formal quotation and current lead times.
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