Pyrethroid, Carbendazim, Organophosphate, Carbamate & Neonicotinoid Antibodies for Food Safety ELISA & LFA IVD Development
Pesticide residue antibodies are monoclonal antibodies raised against pesticide-class haptens conjugated to carrier proteins (BSA or OVA), used to develop competitive ELISA and lateral flow (LFA) rapid tests for crop protection chemical residues in food. Because pesticide active ingredients are small molecules, they are detected using competitive inhibition immunoassay formats rather than sandwich assays: free pesticide in the extracted sample competes with a pesticide-BSA/OVA conjugate for a limited amount of antibody, so higher pesticide concentration produces weaker signal. This format enables ppb-level sensitivity screening across the major pesticide chemical classes — pyrethroids, benzimidazole fungicides, organophosphates, carbamates, and neonicotinoids — that are subject to Maximum Residue Limits (MRLs) in fruits, vegetables, and grains under EU, China GB 2763, and Codex Alimentarius food safety regulations.
Modern crop production relies on multiple pesticide chemical classes, each with a distinct mode of action and regulatory monitoring profile. Pyrethroids are synthetic insecticides derived from naturally occurring pyrethrins, widely used across fruit, vegetable, and grain production for their broad insect-control activity. Organophosphates and carbamates are older but still widely used insecticide classes that inhibit acetylcholinesterase in target pests. Neonicotinoids are a newer systemic insecticide class absorbed into plant tissue, including pollen and nectar — a property that has made their environmental and bee-health impact a subject of ongoing regulatory scrutiny. Benzimidazole fungicides such as carbendazim are used to control fungal disease in a wide range of crops. Because a single food commodity can carry residues from several of these classes simultaneously, food safety laboratories typically deploy a panel of pesticide-class antibodies rather than a single reagent, using rapid immunoassay screening as a first-pass tool ahead of confirmatory GC-MS/MS or LC-MS/MS testing.
Sekbio's pesticide residue antibody panel covers seven pesticide targets across five chemical classes. The flagship Pyrethroids Group Antibody is cross-reactive across eleven pyrethroid compounds with individually documented IC50 values — from cypermethrin at 1 ppb to beta-cyfluthrin at 1,200 ppb — giving kit developers broad pyrethroid-class screening coverage from a single reagent. Carbendazim (CBD), a benzimidazole fungicide, has IC50 0.5 ppb and cross-reacts with benomyl (0.6 ppb) and thiabendazole (10 ppb), two related benzimidazole fungicides. Methyl Parathion, an organophosphate insecticide, has IC50 1 ppb with minimal cross-reactivity to chlorpyrifos, monocrotophos, trichlorfon, and dichlorvos (all under 1%), but notable cross-reactivity to phoxim (100%) and phorate (10%). Chlorpyrifos (CPF), a separate organophosphate insecticide, has IC50 5 ppb. Acetamiprid, a neonicotinoid, has IC50 0.3 ppb. Carbofuran, a carbamate insecticide, has IC50 0.3 ppb and cross-reacts with carbosulfan (30 ppb) and its major metabolite 3-hydroxycarbofuran (50 ppb). Imidacloprid, the most widely used neonicotinoid globally, has IC50 0.1 ppb and cross-reacts with clothianidin (20 ppb) and thiacloprid (30 ppb). Reference price is $895.52/mg for each target, for either the monoclonal antibody or its matching hapten-BSA/OVA conjugate.
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A single Sekbio antibody with documented IC50 values across eleven pyrethroid insecticides — one of the broadest single-reagent pyrethroid panels available for competitive ELISA and LFA development.
| Pyrethroid Compound | IC50 | Relative Sensitivity vs. Cypermethrin |
|---|---|---|
| Cypermethrin | 1 ppb | Most sensitive (1×) |
| Fenpropathrin | 15 ppb | 15× less sensitive |
| Fenvalerate | 20 ppb | 20× less sensitive |
| Esfenvalerate | 40 ppb | 40× less sensitive |
| cis-Cyhalothrin | 40 ppb | 40× less sensitive |
| Bifenthrin | 100 ppb | 100× less sensitive |
| Deltamethrin | 150 ppb | 150× less sensitive |
| Beta-Cypermethrin | 400 ppb | 400× less sensitive |
| cis-Permethrin | 600 ppb | 600× less sensitive |
| Fluvalinate | 1,000 ppb | 1,000× less sensitive |
| Cyhalothrin | 1,000 ppb | 1,000× less sensitive |
| Beta-Cyfluthrin | 1,200 ppb | 1,200× less sensitive |
IC50 values from COA release testing in competitive ELISA format using the Pyrethroids Group Antibody. Reference price $895.52/mg for the monoclonal antibody or matching hapten-BSA/OVA conjugate. Contact info@sekbio.com for full performance data and matrix-specific extraction protocols.
Seven pesticide targets spanning pyrethroid, benzimidazole fungicide, organophosphate, carbamate, and neonicotinoid chemical classes.
| Target Pesticide | Class | IC50 | Key Cross-reactivity | Price (mAb or Conjugate, 1mg) |
|---|---|---|---|---|
| Pyrethroids Group | Pyrethroid (broad) | 1–1,200 ppb (11 compounds) | Cypermethrin, fenpropathrin, fenvalerate, esfenvalerate, cyhalothrin, bifenthrin, deltamethrin, permethrin, fluvalinate, cyfluthrin | $895.52/mg |
| Carbendazim (CBD) | Benzimidazole Fungicide | 0.5 ppb | Benomyl (0.6 ppb), Thiabendazole (10 ppb) | $895.52/mg |
| Methyl Parathion | Organophosphate | 1 ppb | Phoxim (100%), Phorate (10%); <1% CPF/monocrotophos/trichlorfon/dichlorvos | $895.52/mg |
| Chlorpyrifos (CPF) | Organophosphate | 5 ppb | Class-specific | $895.52/mg |
| Acetamiprid | Neonicotinoid | 0.3 ppb | Class-specific | $895.52/mg |
| Carbofuran | Carbamate | 0.3 ppb | Carbosulfan (30 ppb), 3-Hydroxycarbofuran (50 ppb) | $895.52/mg |
| Imidacloprid | Neonicotinoid | 0.1 ppb | Clothianidin (20 ppb), Thiacloprid (30 ppb) | $895.52/mg |
IC50 and cross-reactivity values from COA release testing in competitive ELISA format. Prices are reference list prices per 1 mg of monoclonal antibody or hapten-BSA/OVA conjugate; OEM bulk-scale pricing for 10 mg to gram quantities is quoted on request. Contact info@sekbio.com for full performance data for all targets and matrix-specific extraction protocols.
Sub-ppb sensitivity antibodies validated across five pesticide chemical classes for fruit, vegetable, grain, tea, and honey residue screening.
Sekbio's Pyrethroids Group Antibody is cross-reactive across eleven pyrethroid insecticides with individually documented IC50 values, from cypermethrin at 1 ppb (the most sensitive) to beta-cyfluthrin at 1,200 ppb. Pyrethroids are among the most widely used insecticide classes in global crop production, and covering eleven compounds with a single antibody gives kit developers a commercially significant advantage: broad pyrethroid-class residue screening without sourcing, validating, and stocking eleven separate monoclonal antibodies.
Sekbio's carbendazim antibody (IC50 0.5 ppb) cross-reacts with benomyl (0.6 ppb) and thiabendazole (10 ppb) — both benzimidazole-class fungicides that share structural features with carbendazim. Benomyl in particular degrades to carbendazim in the environment, so combined detection reflects how these residues actually behave in treated crops. This makes the antibody a practical benzimidazole-fungicide-class screening tool for fruits, vegetables, and grains.
Sekbio's methyl parathion antibody (IC50 1 ppb) is highly specific relative to structurally distinct organophosphates — chlorpyrifos, monocrotophos, trichlorfon, and dichlorvos all show under 1% cross-reactivity — while showing 100% cross-reactivity with phoxim and 10% with phorate. In effect, the antibody functions as a combined methyl-parathion/phoxim screening reagent, giving accurate results for these two organophosphates without false positives from the other commonly co-occurring organophosphate residues.
Chlorpyrifos remains one of the most widely monitored organophosphate insecticides globally due to its historical high-volume agricultural use and ongoing regulatory review in multiple jurisdictions. Sekbio's chlorpyrifos antibody (IC50 5 ppb) provides dedicated, class-specific detection for fruit, vegetable, and grain residue screening programs that specifically track chlorpyrifos as a distinct regulatory target from other organophosphates.
Sekbio's carbofuran antibody (IC50 0.3 ppb) detects not only the parent carbamate insecticide but also carbosulfan (30 ppb), a related carbamate that converts to carbofuran in the environment, and 3-hydroxycarbofuran (50 ppb), carbofuran's major metabolite. Covering the parent compound, a precursor, and a metabolite with one antibody reflects how carbofuran-related residues actually appear in treated crops, supporting more complete carbamate residue screening.
Sekbio's imidacloprid antibody (IC50 0.1 ppb, the most sensitive target in this panel) cross-reacts with clothianidin (20 ppb) and thiacloprid (30 ppb), two other neonicotinoid insecticides, giving broad neonicotinoid-class screening coverage from one reagent. Acetamiprid (IC50 0.3 ppb) is available as a complementary, largely class-specific antibody. Neonicotinoids are systemic insecticides absorbed into plant pollen and nectar, making bee exposure and honey contamination a well-documented monitoring priority.
Four of the seven targets on this page — carbendazim, acetamiprid, carbofuran, and imidacloprid — achieve IC50 values at or below 0.5 ppb, providing substantial sensitivity margin for MRL-compliant screening in fruits, vegetables, grains, tea, and honey. Combined with the broader-coverage pyrethroids group, methyl parathion, and chlorpyrifos antibodies, the panel spans both ultra-sensitive single-compound detection and broad multi-compound class screening needs.
MOQ 1 mg per antibody for R&D evaluation. OEM quantities from 10 mg to gram scale available for food safety kit manufacturing. Sekbio's ISO 13485-certified Shenzhen facility provides quality-assured pesticide residue antibody production with lot COA documentation, serving fruit and vegetable exporters, grain processors, tea producers, and honey packers across EU, Chinese, and Southeast Asian markets. Explore Sekbio's related mycotoxin antibodies and phthalate/plasticizer antibody for broader crop and food-contact safety panel development, or contact our team for support.
Sub-ppb competitive immunoassay antibodies for fruit, vegetable, grain, tea, and honey pesticide residue screening at MRL-relevant detection limits.
Fresh fruits and vegetables are the highest-scrutiny commodity category for pesticide residue testing, given direct consumption and typically higher application frequency during the growing season. Sekbio's pyrethroid, carbamate, and organophosphate antibodies — including the 11-compound Pyrethroids Group Antibody, carbofuran, methyl parathion, and chlorpyrifos — support multi-residue competitive ELISA and LFA screening panels for produce exporters, wholesale markets, and import-control testing laboratories operating under EU, China GB 2763, and Codex MRL frameworks.
Rice, wheat, corn, and other grain commodities are tested for pesticide residues both at harvest and during storage and processing, particularly for organophosphate and carbamate insecticides applied during the growing season. Sekbio's chlorpyrifos, methyl parathion, and carbofuran antibodies support grain safety testing programs for domestic quality control and export compliance, where residue levels must remain within destination-market MRLs before shipment.
Tea is one of the most pesticide-residue-scrutinized commodities in global food safety testing, given its status as a widely traded, processed crop consumed as an infusion, which affects how residues transfer into the final beverage. Sekbio's pyrethroid and carbendazim antibodies support tea residue screening programs for growers, processors, and exporters seeking to verify compliance with destination-market pesticide MRLs ahead of shipment.
Neonicotinoid insecticides, including imidacloprid and acetamiprid, are systemic compounds absorbed into plant pollen and nectar, making bee exposure and subsequent honey contamination a well-documented bee-health and honey-quality concern. Sekbio's imidacloprid antibody, cross-reactive with clothianidin and thiacloprid, and acetamiprid antibody support honey and other bee-product testing programs for apiaries, honey packers, and quality control laboratories monitoring neonicotinoid residue levels.
Technical and commercial questions from food safety IVD developers and kit manufacturers building pesticide residue screening assays.
Pesticide residue antibodies are monoclonal antibodies raised against pesticide hapten-carrier protein conjugates (BSA or OVA), used to develop competitive ELISA and lateral flow (LFA) immunoassays. Because pesticide molecules are small, free pesticide in a crop or food sample competes with a pesticide-BSA/OVA conjugate for a limited amount of antibody — higher pesticide concentration produces weaker signal. This competitive format enables sub-ppb to low-ppb sensitivity screening for insecticides, fungicides, and other pesticide classes across fruits, vegetables, grains, tea, and honey, supporting Maximum Residue Limit (MRL) compliance under EU, China GB 2763, and Codex Alimentarius pesticide residue regulations.
Sekbio's Pyrethroids Group Antibody has documented cross-reactivity across eleven pyrethroid insecticides with the following IC50 values: cypermethrin 1 ppb, fenpropathrin 15 ppb, esfenvalerate and cis-cyhalothrin 40 ppb, bifenthrin 100 ppb, deltamethrin 150 ppb, fenvalerate 20 ppb, beta-cypermethrin 400 ppb, cis-permethrin 600 ppb, fluvalinate and cyhalothrin 1,000 ppb, and beta-cyfluthrin 1,200 ppb. This 11-compound cross-reactivity profile allows a single antibody to function as a broad pyrethroid-class screening tool, covering the great majority of pyrethroid insecticides used in crop production, rather than requiring a separate antibody for each individual compound.
Sekbio's methyl parathion antibody (IC50 1 ppb) shows minimal cross-reactivity (under 1%) with structurally distinct organophosphates including chlorpyrifos, monocrotophos, trichlorfon, and dichlorvos — meaning it is largely methyl-parathion-specific relative to those compounds. However, it shows notable cross-reactivity with phoxim (100%) and phorate (10%), both structurally closer organophosphates. In practice, this makes the antibody function as a combined methyl-parathion/phoxim screening reagent, useful where both compounds are of regulatory concern, while remaining specific enough to avoid false positives from chlorpyrifos, monocrotophos, trichlorfon, or dichlorvos residues.
Sekbio's carbofuran antibody (IC50 0.3 ppb) cross-reacts with carbosulfan at 30 ppb and with 3-hydroxycarbofuran at 50 ppb. 3-Hydroxycarbofuran is the major metabolite formed as carbofuran breaks down in plants and soil, and carbosulfan is a related carbamate pesticide that itself converts to carbofuran in the environment. Detecting all three compounds with one antibody is analytically useful for carbamate residue screening programs, since regulatory monitoring is often concerned with total carbofuran-related residue rather than the parent compound alone.
Neonicotinoid insecticides, including imidacloprid and acetamiprid, are systemic pesticides absorbed into plant tissue, including pollen and nectar, making bee exposure and subsequent honey contamination a well-documented bee-health and honey-quality concern. Sekbio's imidacloprid antibody (IC50 0.1 ppb) cross-reacts with clothianidin (20 ppb) and thiacloprid (30 ppb), two other neonicotinoids, giving broad-class neonicotinoid screening coverage. Acetamiprid (IC50 0.3 ppb) is offered as a complementary, largely class-specific reagent. Together these support honey and bee-product testing programs monitoring neonicotinoid residue levels.
Sekbio's pesticide residue antibody panel is validated for competitive ELISA and LFA testing across: fruits and vegetables, for multi-residue screening of pyrethroids, carbamates, and organophosphates; grains such as rice, wheat, and corn; tea, a commodity subject to particularly intensive pesticide residue scrutiny given its status as a processed, widely traded crop; and honey and other bee products, for neonicotinoid contamination monitoring. Contact info@sekbio.com for matrix-specific extraction and cleanup protocol recommendations.
Carbendazim (CBD) is a widely used benzimidazole-class fungicide applied to fruits, vegetables, and grains to control fungal disease. Sekbio's carbendazim antibody (IC50 0.5 ppb) cross-reacts with benomyl (0.6 ppb) and thiabendazole (10 ppb) because both are also benzimidazole fungicides that share core structural features with carbendazim; benomyl, in fact, degrades to carbendazim in the environment and in plant tissue. This cross-reactivity allows the antibody to function as a broad benzimidazole-fungicide-class screening tool rather than a carbendazim-only reagent.
MOQ is 1 mg per antibody for R&D evaluation and assay development. Reference price is $895.52 per mg across all seven pesticide targets on this page — Pyrethroids Group, Carbendazim, Methyl Parathion, Chlorpyrifos, Acetamiprid, Carbofuran, and Imidacloprid — covering either the monoclonal antibody or its matching hapten-BSA/OVA conjugate (1 mg each). OEM quantities from 10 mg to gram scale are available for kit manufacturing, with bulk pricing quoted on request. Contact info@sekbio.com for a formal quotation and current lead times.
Request pesticide residue antibody evaluation kits, hapten conjugates, or discuss OEM food safety ELISA and LFA kit development support.