Advanced blocking solution that eliminates heterophilic antibody interference, ensuring accurate and reliable immunological assay results for all diagnostic applications.
Heterophilic antibodies are antibodies that have two or more different antibody structures, produced by two or more different clones of B cells. These antibodies can cause false positive or false negative results in immunological assays.
Heterophilic antibodies in human serum contain two or more different heavy or light chains, produced through homologous or heterologous recombination. They have multiple subtypes and specificities.
These antibodies can cause false positive or false negative interference in immunological assays, significantly affecting the accuracy of diagnostic results.
Adding Heterophilic Blocking Reagent (HBR) to immunological assays effectively eliminates the interference of heterophilic antibodies, ensuring reliable results.
HBR is a liquid reagent designed to be added to immunological assays to eliminate heterophilic interference (both false positive and false negative) in certain human samples.
Heterophilic samples refer to serum or plasma samples with specific antibodies that can bind to the animal antibodies used in immunoassays. The interference is commonly reported as false positives, but false negatives have also been reported. Common heterophilic antibodies include Human Anti-mouse Antibody (HAMA) and rheumatoid factor (RF).
How it works: Blockers can bind to non-specific reactive substances in the reagents, thereby eliminating false positive results. They competitively bind to these substances, preventing them from binding to non-specific substances in the sample.
Analyte bridges Capture and Detect antibodies, producing a true positive signal.
→ ACCURATE RESULTHeterophilic antibody (HA) directly bridges Capture and Detect antibodies — no analyte needed. A false signal is generated.
→ FALSE POSITIVEHBR sequesters the heterophilic antibody (HA) into an inert complex. The sandwich assay proceeds normally with the true analyte.
→ ACCURATE RESULTFigure: How HBR blocks heterophilic antibody interference
Understanding the mechanism of heterophilic interference and how HBR eliminates it
In a normal sandwich immunoassay, the analyte is bound by the antibody, resulting in a positive result.
Heterophilic antibodies can cause false positive results by binding to assay antibodies without analyte presence.
HBR actively binds to human heterophilic antibodies, creating steric hindrance and preventing interference.
With HBR, the assay delivers accurate, reliable results by eliminating heterophilic interference.
Our HBR product offers superior blocking performance compared to traditional blockers
Only a small amount of HBR is required for effective blocking without reducing the detection signal, significantly saving your costs.
Our HBR can block all subtypes of anti-mouse monoclonal antibodies (such as anti-mouse IgG1, anti-mouse IgG2, etc.).
Our HBR is applicable for all immunoassay types and is not limited to certain specific assays, offering maximum flexibility.
Evaluation experiments demonstrate that SEKBIO's blocker outperforms Top brand's blocker in eliminating heterophilic interference.
Evaluation utilizing a fluorescent lateral flow assay with six clinical samples
Fluorescent lateral flow assay, six RF-positive clinical samples. T/C ratio — lower is better. Best blocking result per sample highlighted.
| False Positive Clinical Sample |
T/C Value |
No Blocker Added — Reference |
Top Brand's Blocker HBR-2 |
SEKBIO's Blocker S01-blocker-01 |
SEKBIO's Blocker S01-blocker-02 |
|---|---|---|---|---|---|
| No. 13# | T/C | 3.33 | 2.84 | 2.98 | 1.45 |
| No. 15# | T/C | 8.49 | 1.4 | 1.38 | 0.33 |
| No. 25# | T/C | 4.89 | 2.04 | 2.3 | 2.13 |
| No. 15# | T/C | 4.63 | 0.94 | 0.93 | 0.66 |
| No. 46# | T/C | 3.72 | 1.91 | 1.93 | 1.41 |
| No. 49# | T/C | 3.07 | 3.27 | 3.43 | 0.66 |
Note: Lower T/C ratio = better blocking performance. SEKBIO's S01-blocker-02 achieves the lowest T/C in 5 of 6 samples tested, outperforming Top Brand's HBR-2 across the clinical panel.
Contact us today to request a quote or learn more about SEKBIO Heterophilic Blocking Reagent