IVD Raw Materials · Viral Antigens & Antibodies

Ebola Antibodies
& Recombinant Antigens

Comprehensive portfolio of anti-Ebola monoclonal antibodies and recombinant GP/VP40/NP proteins for Ebola virus diagnostic assay development — covering Zaire and Sudan strains.

14 Products Zaire & Sudan GP / VP40 / NP ELISA / LFIA / CLIA Mammalian / Insect / Prokaryotic
14
Products (Abs + Ags)
2
Virus Strains Covered
3
Target Proteins (GP/VP40/NP)
IVD
For Diagnostic Use

Key Highlights for IVD Developers

  • Complete pairing portfolio: Coating-grade and labeling-grade anti-Ebola monoclonal antibodies enable sandwich assay development with matched pairs.
  • Dual-strain coverage: Recombinant antigens available for both Zaire Ebolavirus (most virulent) and Sudan Ebolavirus, supporting regional diagnostic needs.
  • Three expression systems: Mammalian cell (native glycosylation), insect cell (high-yield), and prokaryotic (cost-effective) expression options to match assay sensitivity requirements.
  • Three key targets: GP (entry glycoprotein), VP40 (matrix protein), and NP (nucleoprotein) — covering both early and late-stage infection detection.
  • Multi-platform validation: Antibodies validated for ELISA, lateral flow (LFIA), and CLIA; recombinant antigens validated as coating antigens and assay standards.

Ebola Virus in IVD: Background

Ebola virus disease (EVD) is a severe, often fatal illness in humans caused by Ebola virus, a member of the Filoviridae family. The Zaire Ebolavirus strain has the highest case fatality rate (up to 90%), while Sudan Ebolavirus also causes significant outbreaks. Rapid and accurate diagnosis is critical for outbreak containment.

Key Diagnostic Targets

  • GP (Glycoprotein): The surface glycoprotein responsible for viral entry. Most immunoassays target GP for its strong immunogenicity and surface accessibility.
  • NP (Nucleoprotein): The most abundant viral protein, highly conserved across strains. Anti-NP antibodies emerge early in infection, making NP an excellent target for early detection.
  • VP40 (Matrix Protein): Highly conserved among filoviruses. Useful for broadly reactive assays and as a secondary confirmatory target.

Expression System Selection

Mammalian cell expression (Ebo-1, Ebo-2) produces proteins with native human-like glycosylation — ideal for high-sensitivity immunoassays. Insect cell expression (Ebo-3, Ebo-4) offers a balance of proper folding and cost-effectiveness. Prokaryotic expression (Ebo-5, Ebo-6) produces non-glycosylated proteins suitable for specific epitope detection and as cost-effective assay standards.

Antibodies Overview
Products6 Monoclonal Antibodies
TargetsNP, VP40
FormatCoating / Labeling Grade
HostMouse (mAb & Recombinant)
Validated PlatformsELISA, LFIA, CLIA
Recombinant Antigens Overview
Products8 Recombinant Proteins
TargetsGP, VP40, NP
StrainsZaire, Sudan
ExpressionMammalian / Insect / Prokaryotic
ApplicationCoating Ag / Standard

Monoclonal Antibodies

Mouse monoclonal and recombinant anti-Ebola antibodies for sandwich immunoassay development.

Cat No. Product Name Target Type Application
Ebo-1M Mouse Anti-Ebola NP mAb NP (Nucleoprotein) Mouse mAb Chromatography Coating
Ebo-2MB Recombinant Mouse Anti-Ebola NP mAb NP (Nucleoprotein) Recombinant Mouse mAb Chromatography Labeling
Ebo-3M Mouse Anti-Ebola VP mAb VP40 (Matrix Protein) Mouse mAb Immunoassay
Ebo-4M Mouse Anti-Ebola VP mAb VP40 (Matrix Protein) Mouse mAb Immunoassay
Ebo-4MB Recombinant Mouse Anti-Ebola VP mAb VP40 (Matrix Protein) Recombinant Mouse mAb Chromatography Labeling
Ebo-5MB Recombinant Mouse Anti-Ebola VP mAb VP40 (Matrix Protein) Recombinant Mouse mAb Chromatography Coating

Coating-grade antibodies are optimized for solid-phase immobilization. Labeling-grade antibodies are optimized for conjugation to colloidal gold, fluorescent beads, or enzymes.

Recombinant Antigens

Recombinant Ebola virus proteins expressed in mammalian, insect, and prokaryotic systems.

Cat No. Product Name Strain Target Protein Expression System
Ebo-1 Recombinant Zaire Ebolavirus GP Protein Zaire Zaire GP (Glycoprotein) Mammalian Cell
Ebo-2 Recombinant Sudan Ebolavirus GP Protein Sudan Sudan GP (Glycoprotein) Mammalian Cell
Ebo-3 Recombinant Zaire Ebolavirus GP Protein Zaire Zaire GP (Glycoprotein) Insect Cell
Ebo-4 Recombinant Sudan Ebolavirus GP Protein Sudan Sudan GP (Glycoprotein) Insect Cell
Ebo-5 Recombinant Zaire Ebolavirus VP40 Protein Zaire Zaire VP40 (Matrix Protein) Prokaryotic (E. coli)
Ebo-6 Recombinant Sudan Ebolavirus VP40 Protein Sudan Sudan VP40 (Matrix Protein) Prokaryotic (E. coli)
Ebo-7 Recombinant Zaire Ebolavirus NP Protein Zaire Zaire NP (Nucleoprotein)
Ebo-8 Recombinant Sudan Ebolavirus NP Protein Sudan Sudan NP (Nucleoprotein)

Mammalian cell expression provides native glycosylation. Insect cell expression offers high yield with partial glycosylation. Prokaryotic expression is cost-effective for standard/control use.

Applications in Ebola IVD Development

Sekbio's Ebola antibodies and antigens support multiple diagnostic assay formats.

Sandwich ELISA

Pair coating-grade (Ebo-1M, Ebo-5MB) and labeling-grade (Ebo-2MB, Ebo-4MB) antibodies for sensitive Ebola antigen detection in patient serum or plasma.

Rapid Test (Lateral Flow)

Anti-Ebola antibodies conjugated to colloidal gold or fluorescent beads for point-of-care Ebola virus antigen detection in resource-limited settings.

CLIA / Automated immunoassay

High-sensitivity chemiluminescent immunoassays using Sekbio antibodies for automated Ebola diagnostics in clinical laboratories.

Multiplex Pathogen Panels

Recombinant GP and NP proteins (Ebo-1, Ebo-7) as positive controls or calibration standards in multiplex PCR/antigen panels for febrile illness diagnosis.

Assay Development & Optimization

Recombinant antigens as coating antigens and assay standards for optimizing capture antibody concentration, block conditions, and evaluating assay sensitivity.

Surveillance & Research

Antibodies and antigens for Ebola virus research, epidemiological surveillance, and vaccine efficacy studies in BSL-2/BSL-3 research laboratories.

Frequently Asked Questions

What Ebola virus strains do Sekbio antigens cover?
Sekbio provides recombinant antigens for both Zaire Ebolavirus (the most virulent and common strain, Cat No. Ebo-1/3/5/7) and Sudan Ebolavirus (Ebo-2/4/6/8). Target proteins include GP (Glycoprotein), VP40 (matrix protein), and NP (nucleoprotein), expressed in mammalian cells, insect cells, or prokaryotic systems depending on the catalog number.
Which assay platforms are Sekbio Ebola antibodies validated for?
Sekbio's anti-Ebola monoclonal antibodies (Ebo-1M through Ebo-5MB) are validated for use in sandwich ELISA, lateral flow immunoassay (LFIA), and CLIA platforms. Coating-grade antibodies (Ebo-1M, Ebo-5MB) are optimized for solid-phase immobilization; labeling-grade antibodies (Ebo-2MB, Ebo-4MB) are optimized for conjugation to colloidal gold, fluorescent beads, or enzymes.
What is the difference between mammalian, insect cell, and prokaryotic expression for Ebola antigens?
Mammalian cell expression (Ebo-1, Ebo-2) produces proteins with native post-translational modifications (glycosylation), ideal for high-sensitivity immunoassays. Insect cell expression (Ebo-3, Ebo-4) offers high yield with partial glycosylation — a cost-effective alternative. Prokaryotic expression (Ebo-5, Ebo-6, VP40 series) produces non-glycosylated proteins suitable for specific epitope detection and as assay standards.
How do I choose between anti-NP and anti-VP40 antibodies for assay development?
NP (nucleoprotein) is the most abundant and immunodominant Ebola protein, making anti-NP antibodies ideal for early detection and high-sensitivity assays. VP40 (matrix protein) is also highly conserved across strains and useful for broadly reactive assays. For maximum sensitivity, a sandwich assay pairing an anti-NP capture antibody with an anti-VP40 detection antibody (or vice versa) can be considered.
How do I request a sample or technical datasheet for Ebola products?
Please contact our technical team at info@sekbio.com with your catalog number(s) of interest, intended application, and required quantity. We provide evaluation samples and full technical support for assay development projects.

Accelerate Your Ebola Diagnostic Assay Development

Our technical team can help you select the right antibody pairs and recombinant antigens for your assay platform and target strain. Request evaluation samples and technical support today.

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