LPS Antigen & Recombinant Antigen — high-purity components validated for Brucellosis ELISA and immunoassay development
Brucellosis is one of the world's most widespread zoonotic diseases, caused by gram-negative bacteria of the genus Brucella. It affects livestock (cattle, sheep, goats, pigs) and transmits to humans through contact with infected animals or consumption of unpasteurized dairy products. Globally, an estimated 500,000 new human cases are reported annually.
Accurate serological diagnosis of Brucellosis relies on detecting anti-Brucella antibodies (IgM, IgG) in patient serum. The choice of antigen critically determines assay sensitivity and specificity:
The cell-wall lipopolysaccharide (LPS) of smooth Brucella species is the dominant immunogenic component during natural infection. LPS-based antigens are the traditional standard for Brucellosis serology, offering high sensitivity for detecting antibodies in both acute and chronic phases.
Recombinant Brucella antigens expressed in E. coli provide a safer, chemically defined alternative. They offer improved specificity over LPS antigens, are free of biohazardous bacterial material, and support highly reproducible manufacturing — making them increasingly preferred for modern IVD kit development and automated immunoassay platforms.
Choose the antigen format best suited to your assay platform and development requirements.
| Parameter | BRU-LPS-02 · LPS Antigen | BRU-01 · Recombinant Antigen |
|---|---|---|
| Description | Lipopolysaccharide extracted from Brucella abortus | Prokaryotic recombinant (E. coli) expressed protein |
| Antigen Type | Native LPS | Recombinant protein (E. coli) |
| Form | Liquid | Liquid |
| Purification Method | — | Affinity chromatography |
| Purity | ≥90% | ≥90% |
| Buffer System | 0.05M Carbonate buffer, pH 9.6 | Tris buffer |
| Appearance | Clear or slightly opalescent solution | Clear or slightly opalescent solution |
| Validation Method | Indirect ELISA | Indirect ELISA |
| Storage Temperature | 2–8°C (do not freeze) | Short-term: −20°C; Long-term: −70°C |
| Application | Immunoassay | Immunoassay |
| Note | For diagnostic use only | For diagnostic use only |
Sekbio's Brucella antigens are designed for use across multiple immunoassay platforms.
Use as coating antigen in indirect ELISA for IgM and IgG serodiagnosis. High purity minimizes non-specific binding and improves signal-to-noise ratio in human and animal serum panels.
High-purity antigens are compatible with colloidal gold and fluorescent bead conjugation for lateral flow assay development, enabling rapid point-of-care Brucellosis detection.
Brucellosis is a major concern in livestock management globally. Sekbio's antigens support veterinary diagnostic kit development for cattle, sheep, goat, and swine testing programs.
Validated antigens support large-scale Brucellosis surveillance programs in endemic regions, suitable for both clinical laboratory automation and field-deployable assay formats.
Standardized expression and purification workflows ensure consistent supply of GMP-compatible raw materials for commercial diagnostic kit manufacturers.
Suitable for research laboratories developing or benchmarking Brucellosis serology assays, evaluating coating concentrations, blocking conditions, and cross-reactivity panels.
Our technical team can help you select the right antigen format, optimize coating conditions, and accelerate your diagnostic kit to market.