Human Chorionic Gonadotropin (Total HCG β)
The performance data of the Total HCG β Rapid Quantitative Test Kit(CLIA)
Manufacturer | SEKBIO |
Parameter | Total HCG β |
Linearity | 0.500-10000 mIU/mL |
Detection limit | 0.500 mIU/mL |
benchmark product | Beckman |
Test principle and procedure | FSH: Add 30 μl of sample + 30 μl of Ra+ 30 μl of Rd incubation for 10 minutes. Wash with 200 μl of washing solution. Finally, add 100 μl of A + 100 μl of B. |
Six point calibration test results
Sample Concentration( mIU/ml ) |
RLU | Average | CV | S/N | ||
0.00 | 247 | 238 | 153 | 213 | 24.39% | / |
5.79 | 7,950 | 7,389 | 7,355 | 7,565 | 4.42% | 35.57 |
622.95 | 722,593 | 697,041 | 682,661 | 700,765 | 2.89% | 3295.13 |
1258.42 | 1,259,862 | 1,240,478 | 1,358,218 | 1,286,186 | 4.91% | 6047.90 |
5998.99 | 4,834,497 | 5,537,553 | 5,042,930 | 5,138,327 | 7.03% | 24161.41 |
11406.29 | 8,165,598 | 8,137,136 | 8,081,462 | 8,128,065 | 0.53% | 38219.74 |
Precision
Sample | RLU | Concentration | Sample | RLU | Concentration |
685.28 | 719.1 | 704,736 |
6517.82 | 6,824 | 5,365,410 |
713.3 | 699,429 | 6,441 | 5,110,212 | ||
714.1 | 700,156 | 6,171 | 4,928,047 | ||
670.0 | 659,635 | 6,864 | 5,391,909 | ||
714.0 | 700,051 | 6,548 | 5,182,126 | ||
725.7 | 710,760 | 6,794 | 5,345,787 | ||
672.4 | 661,800 | 6,649 | 5,249,877 | ||
705.7 | 692,437 | 6,441 | 5,110,737 | ||
658.4 | 648,964 | 6,669 | 5,262,868 | ||
711.4 | 697,639 | 6,394 | 5,078,994 | ||
AVE | 700.41 | 687,560.70 | AVE | 6,579.39 | 5,202,596.70 |
SD | 23.91 | 21,977.81 | SD | 221.16 | 147,861.12 |
CV | 3.4% | 3.2% | CV | 3.4% | 2.8% |
Limit of detection
Sample | RLU | Concentration | ||
Ag Diluent | 207 | 114 | 0.005 | 0.003 |
317 | 144 | 0.009 | 0.003 | |
331 | 127 | 0.009 | 0.002 | |
307 | 146 | 0.008 | 0.003 | |
166 | 170 | 0.002 | 0.004 | |
163 | 155 | 0.003 | 0.004 | |
225 | 179 | 0.006 | 0.004 | |
199 | 99 | 0.005 | 0.001 | |
177 | 157 | 0.003 | 0.002 | |
204 | 184 | 0.005 | 0.004 | |
AVE | 189 | 0.051 | ||
SD | 64 | 0.025 | ||
AVE+2*SD | 0.101 | |||
Linear
Sample( mIU/ml ) | Dilution ratio | Concentration value | Average | ||
0.00 | 0.0 | 0.0 | 0.0 | 0.0 | 0 |
2275.59 | 0.2 | 2,052.0 | 2,000.3 | 1,951.4 | 2,001 |
4551.18 | 0.4 | 4,219.2 | 4,556.2 | 4,665.2 | 4,480 |
6826.76 | 0.6 | 6,836.6 | 6,895.1 | 6,780.0 | 6,837 |
9102.35 | 0.8 | 9,901.1 | 9,198.6 | 9,144.3 | 9,415 |
11377.94 | 1.0 | 11,456.8 | 11,396.8 | 11,280.2 | 11,377.94 |
Method Comparison
- Cardiac Markers
-
Tumor Marker
- CA50
-
CA125
- CA242
-
CA15-3
- CA19-9
- CA72-4
-
Pepsinogens I (PGI)
-
Human Epididymis 4 (HE4)
- Prostate-Specific Antigen (PSA)
- Squamous Cell Carcinoma (SCC)
- Neuron-Specific Enolase (NSE)
- Cytokeratin 19 Fragment (CYFRA21-1)
- Human Progastrin-releasing Peptide (ProGRP Tumor Marker)
- Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA II Tumor Marker)
- Alpha-fetoprotein(AFP)
- Inflammatory Marker
- Infectious Disease
- Hormones
- Thyroid Function
- Glucose Metabolism
- Bone Marker
- Others
-
Heterophilic Blocking Reagent
- Animal Diagnostics
