LH
LH
LH(Luteinizing Hormone) is a gonadotropin hormone produced by the anterior pituitary gland. It plays a vital role in the regulation of reproductive processes in both males and females. In females, LH works with FSH to regulate ovulation and the formation of the corpus luteum. In males, LH stimulates testosterone production in the testes.
Key Functions:
1. In Men:
- Stimulates testosterone production in the testes by acting on the interstitial cells.
- Promotes the development of sperm-producing cells (germinal epithelium).
2. In Women:
- Stimulates the maturation of ovarian follicles.
- Triggers ovulation and the formation of the corpus luteum.
- Stimulates estrogen production by the ovaries.
Normal LH Values:
1. In Men:
- Normal range: 1.5 IU/L to 9.3 IU/L.
2. In Women:
- Follicular Phase: 1.9 IU/L to 12.5 IU/L.
- Ovulation: 8.7 IU/L to 76.3 IU/L.
- Luteal Phase: 0.5 IU/L to 16.9 IU/L.
- Menopause: 15.9 IU/L to 54 IU/L.
- Pregnancy: 0 IU/L to 1.5 IU/L.
Clinical Applications:
1. In Women:
- Ovulation Testing: LH helps in the assessment of ovulation by triggering the release of the egg from the follicle.
- Gonadal Function: LH levels are used to assess the function of the ovaries, including ovulatory disorders and conditions like polycystic ovary syndrome (PCOS).
2. In Men:
- Testicular Function: LH plays a key role in stimulating testosterone production and sperm production, making it an important marker for evaluating male gonadal function.
- Infertility: LH testing can help diagnose conditions related to male infertility, including problems in the hypothalamic-pituitary-gonadal axis.
The performance data of the LH Rapid Quantitative Test Kit(CLIA)
Manufacturer | SEKBIO |
Parameter | LH |
Linearity | 0.1-200 mIU/mL |
Detection limit | 0.1 mIU/mL |
benchmark product | Roche |
Test principle and procedure | PG Ⅱ: Add 35 μl of sample + 30 μl of Ra +30 μl of Rd and incubation for 15 minutes. Wash with 200 μl of washing solution. Finally, add 100 μl of A + 100 μl of B. |
Analytical sensitivity
Sample Concentration(mIU/mL) | RLU | Average | CV | S/N | |
219.85 | 3,101,159 | 3,280,048 | 3,190,604 | 3.96% | 7169.9 |
188.08 | 2,780,782 | 2,679,790 | 2,730,286 | 2.62% | 6135.5 |
78.25 | 957,837 | 924,700 | 941,269 | 2.49% | 2115.2 |
17.68 | 107,259 | 112,512 | 109,886 | 3.38% | 246.9 |
4.03 | 12,231 | 12,125 | 12,178 | 0.62% | 27.4 |
0.14 | 422 | 468 | 445 | 7.31% | 1.0 |
Method Comparison
